神秘果中齐墩果酸和五环三萜含量的测定

Determination of the Content of Oleanolic Acid and Pentacyclic Triterpenes from Synsepalum Dulcificum Daniell

微波提取神秘果中齐墩果酸和五环三萜,并用HPLC测定齐墩果酸含量,比色法测定五环三萜的含量。结果表明：齐墩果酸在0.01-0.05 mg/mL范围内线性关系良好,回归方程为：Y=5.8672E-8X-2.908 07E-4,RSD=1.33%,相关系数r=0.995 72,含量为0.03 mg/g。五环三萜在0.1-0.6 mg/mL范围内线性关系良好,A=2.92C＋1.272,相关系数r=0.990 04,含量为0.9 mg/g。试验建立的两种方法操作简便、准确、快速,适合神秘果中齐墩果酸及五环三萜含量的测定。

Microwave extraction was used to extract oleanolic acid and pentacyclic triterpenes from Synsepalum dulcificum Daniell. The content of oleanolic acid was determined by high performance liquid chromatography. The content of pentacyclic triterpenes was determined by colorimetric determination. The experimental results indicated that when concentration of oleanolic acid rang from 0.01 mg/mL to 0.05 mg/mL, the regressive equation of the calibration graph was Y=5.867 2E-8X-2.908 07E-4, RSD=l.33%, r square value was 0.990 04, the content was 0.03 mg/mL. When concentration of pentacyclic triterpenes rang from 0.1 mg/mL to 0.6 mg/mL, the regressive equation of the calibration graph was A=2.92C＋1.272, r square value was 0.990 04, the content was 0.9 mg/mL. The method was simple, rapid, and accurate to be used in determination of oleanolic acid and pentacyclic triterpenes from Synsepalum dulcificum Daniell.