维药斯亚旦化学成分研究

Study on chemical constituents of Nigella glanduliferaFreyn et Sint.

目的研究维药斯亚旦的化学成分。方法采用正相硅胶、ODS、Sephadex LH-20等柱色谱以及高效制备液相等手段进行分离纯化,并用化学方法和光谱方法鉴定了化合物的结构。结果从斯亚旦中分离鉴定了10个化合物,分别为:五加苷K(1)、常春藤皂苷元-3-O-α-L-鼠李糖基(1→2)-α-L-吡喃阿拉伯糖苷(2)、常春藤皂苷元-3-O-β-D-吡喃木糖基(1→3)-α-L-鼠李糖基(1→2)-α-L-吡喃阿拉伯糖苷(3)、quercetin-3-[6-ferulyl-β-D-glucopyranosyl(1→2)-β-D-glucopyranosyl(1→2)-β-D-glucopyrano-side](4)、quercetin-3-sophorotriosides(5)、quercetin-3-glucosyl(1→2)-galactosyl(1→2)-glucoside(6)、quercetin-3-(6-feruloylglu-cosyl)(1→2)-galactosyl(1→2)-glucoside(7)、3-O-α-L-吡喃鼠李糖-(1→2)-α-L-吡喃阿拉伯糖常春藤皂苷元-28-O-α-L-吡喃鼠李糖基(1→4)-β-D-吡喃葡萄糖基(1→6)-β-D-吡喃葡萄糖(8)、3-O-β-D-吡喃葡萄糖基(1→6)-吡喃鼠李糖基-(1→2)-α-L-吡喃阿拉伯糖常春藤皂苷元-28-O-α-L-吡喃鼠李糖基(1→4)-β-D-吡喃葡萄糖基(1→6)-β-D-吡喃葡萄糖苷(9)和芦丁(10)。结论化合物1,4,5为首次从黑种草属中分离得到,化合物6为首次从该种中分离得到。

Abstract：Objective To study the constituents of Nigella glandulifera Freyn et Sint.. Methods The compounds were isolated and obtained by chromatography including SiP2 gel,ODS and Sephadex LH-20. The structures were determined by chemical methods including NMR spectrum. Results The obtained compounds were eleutheroside K（1）, hederagenin-3-0.a-L-rhamnopyranosyl（ 1→ 2 ）-α-L-arabinopyranoside （ 2 ） , hederagenin-3-O-β-D-xylopyranosyl（ 1→ 3 ）-α-L-rhamnopyranosyl [ 1→ 2 ）-0rL-arabinopyranoside （ 3 ）, quercetin-3-[ 6-ferulyl-fl-D-glucopyranosyl （1 → 2 ）-β-D-glucopyranosyl （ 1 →2 ）-β-D-glucopyranoside] （ 4 ）, quereetin-3-sophorotrio- sides （ 5 ）, quercetin-3-glucosyl （ 1 → 2 ）-galactosyl （1 → 2 ）-glucoside （ 6 ）, quercetin-3- （ 6-feruloylglucosyl ） （ 1 →2 ）-galactosyl （ 1 → 2 ）- glucoside （ 7 ）, 3-O- [ a-L-rhamnopyranosyl- （1→2）-α-L-arabinpyranosyl-28-O- [α-L-rhamnopyranosyl- （ 1→ 4 ） -β-D-glucopyranosyl-（1→-）-β-D-glucopyranosyl]. hederagenin（ 8 ）, 3-0. [fl-D-glucopyranosyl- （1→4）-α-D-rhamnopyranosyl-（1→4）-α-D-arabinpyranosyl]- 28-O. [α-L-rhamnopyranosy-（1→4）-β-D-glucopyranosyl-（1→4）-β-D-glucopyranosyl ] hederagenin （ 9 ）, and rutin （ 10 ）. Conclusion Compounds 1,4,5 ware obtained from the genera for the first time. Compound 6 was obtained from the genus for the first time.