急性期脑出血大鼠不同时间内给予三七治疗的作用对比研究

Comparative Study on the Effect of Pseudo-ginseng on Acute Cerebral Hemorrhage Afrats in Different Time

目的急性期脑出血大鼠不同时间给予三七治疗,观察治疗后脑血肿、脑水肿及出血半暗带脑组织Bcl-2表达情况,评价不同时间给药的差异。方法将210例应用自体血法注射建立脑出血大鼠模型随机分为7组:即刻给药、第1天后给药、第2天后给药、第3天后给药、第5天后给药、第7天后给药组及对照组,每组30例;术后第8天观察脑水肿、脑血肿情况及出血半暗带脑组织Bcl-2表达情况。结果⑴与对照组相比:即刻给药治疗组脑含水量偏高(P<0.01);其他各时点给药组大鼠脑含水量、血肿体积明显减小(均P<0.05),尤以第3天后给药组降低最明显(P<0.01)。②与对照组相比,各时间点给药组脑组织Bcl-2表达均有增高(均P<0.01),以第3天后给药组最高(P<0.01)。结论三七在一定时间窗内给药可减轻急性期脑出血大鼠脑水肿,减少血肿体积,可以上调Bcl-2蛋白表达抑制脑组织细胞凋亡。选择适当的时间窗应用三七活血化淤类中药治疗急性期脑出血是安全的,有效的。

Objective To investigate the influence of radix pseudo-ginseng on Bcl-2 expression of acute intracerebral hemorrhage rats and application time. Methods Cerebral hemorrhage rats were established by autologous blood injection;Depending on the drug administration time after autologous blood injection,the rats were randomly divided into six groups,namely the immediate drug administration group,the first day group,the second day group,the third day group,the fifth day group,seventh day group after modeling and control group.8 days after autologous blood injection,cerebral edema,cerebral hematoma,bleeding penumbra and the expression of Bcl-2 were investigated. Results ①Compared with the control group,mortality of the immediate drug administration group was increased and edema volume was elevated（P0.01）,the edema volume and hematoma volume of other groups were deceased（P0.05）,especially in the third day group（P0.01）.② Compared with the control group,the expression of Bcl-2 in treatment groups was increased（P0.01）,and the third day group was the highest（P0.01）. Conclusion Radix pseudo-ginseng can relieve brain edema,decrease hematoma volume,upregulate Bcl-2 protein and inhibit apoptosis of brain tissue within a certain time window in acute cerebral hemorrhage.