Foxp3以及TGF-β1对胚胎反复种植失败的影响

Foxp3 and TGF-β1 on the impact of embryo repeated implantation failure

目的:研究黄体中期和取卵后第6天外周血Foxp3、TGF-β1 mRNA的表达情况,探究二者表达对胚胎反复种植失败的影响。方法:以反复种植失败16例为实验组,以同期接受IVF-ET第1周期并妊娠的25例为对照组。分别抽取黄体中期(LH)和取卵后(OPU)第6天的空腹静脉血各2ml(要求P≥5ng/ml),采用RT-PCR法检测Foxp3和TGF-β1 mRNA水平。结果:反复移植失败组外周血d(LH+6)、d(OPU+6)Foxp3 mRNA表达和对照组相比,差异有统计学意义(分别为0.561±0.029 vs 0.718±0.0291,0.594±0.034 vs 0.727±0.030,P均<0.05)。反复种植失败组外周血d(LH+6)、d(OPU+6)TGF-β1 mRNA水平与对照组相比,差异均有统计学意义(分别为0.638±0.056 vs 0.849±0.056、0.731±0.035 vs 0.837±0.052,P均<0.05)。结论:反复种植失败患者外周血Foxp3及免疫抑制因子TGF-β1的表达降低,可能是反复种植失败免疫性不孕的原因。

Objective：To investigate the reason of repeated plant failures by studying the Foxp3,TGF-β1 mRNA expression in the embryonic at the period of luteal phase and the ovum after six days.Methods：Sixteen patients with repeated cultivation failures were recuited 25 cases who received the first cycle of IVF-ET treatment services at the same period were recuited as controls.The Foxp3 and TGF-β1 mRNA were estimated by detecting the fasting blood 2ml collected when the P value≥5ng/ml（the luteal phase）and the ovum after six days via RT-PCR.Results：Compared with the controls,the d（LH＋6）,d（OPU＋6）Foxp3 in peripheral blood of the repeated graft failure showed statistically significant difference（P0.05）（0.561±0.029 vs 0.718±0.0291,0.594±0.034 vs 0.727±0.030）.The expression of the d（LH＋6）,d（OPU＋6）TGF-β1 mRNA in peripheral blood of the repeated graft failure and the controls are 0.638±0.056 vs 0.849±0.056,0.731±0.035 vs 0.837±0.052,also showed statistical difference（P0.05）.Conclusion：The reduced mRNA expression of the Foxp3 and TGF-β1 in the peripheral blood of the repeated cultivation patients may be the mainly reasons of the failure of immune infertility.