人乳头瘤病毒生物状态和人端粒酶RNA组分基因扩增与宫颈病变的关系

Relationship of biological status of HPV and human TERC gene amplification with cervical lesions

目的:探讨人乳头瘤病毒(HPV)生物状态和人端粒酶RNA组分(hTERC)基因扩增与子宫颈病变之间的关系。方法:应用原位杂交(ISH)和荧光原位杂交(FISH)方法,分别检测114例宫颈石蜡包埋组织HPV生物状态及hTERC基因的异常扩增。结果:在非CIN、CINⅠ、CINⅡ、CINⅢ、宫颈癌宫颈组织标本中hTERC基因表达率分别为2.78%、10.00%、50.00%、78.37%和86.67%,差异有统计学意义(χ2=55.56,P<0.001)。随着病变程度升高,HPV整合发生率逐渐升高(0.0%、10.00%、18.75%、72.97%、80.00%)。不同程度的宫颈病变HPV整合率有显著差异(χ2=52.49,P<0.001)。其中,非CIN组与CINⅢ、宫颈癌组之间差异有统计学意义,CINⅠ与CINⅢ、宫颈癌组之间差异有统计学意义,CINⅡ与CINⅢ、宫颈癌组之间差异有统计学意义。HPV DNA整合情况和hTERC基因扩增情况一致,用两种方法预测宫颈癌的效果一致。结论:HPV生物状态和TERC基因扩增与子宫颈病变的发生发展相关。HPV感染与HPV整合、端粒酶激活有时序性差异,但HPV整合和hTERC基因扩增的时序性和关联性有待进一步探讨。

Objective：To explore the strongly associated events of genomic integration of oncogenic HPV and gain of the human telomerase gene TERC in the progression of uterine cervical dysplasia to invasive cancer.Methods：In situ hybridization（ISH） and fluorescence in situ hybridization（FISH）was used to detect the biological status of HPV and amplification of hTERC of cervical epithelial cells in 114 cases.Results：While 2.78% of the non-CIN,10.00% of the CINⅠ,50.00% of 78.37% of and 86.67% of the squamous cervical cancer showed extra copies of TERC gene copy numbers,the results were compared with the pathologic diagnosis.（χ2=55.56,P0.001）.With aggravated cervical lesions,the incidence of HPV integrated gradually increased（0.0%,10.00%,18.75%,72.97%,80.00%）.The precalence of integrated from varying degrees of cervical lesions with significant differences between any 2 groups（χ2 =52.49,P0.001）.The incidence of HPV integrated between the non-CIN and the CINⅢ or the squamous cervical cancer was significant differences.The incidence of HPV integrated between the CINⅠand the CINⅢ or the squamous cervical cancer was significant differences.The incidence of HPV integrated between the CINⅡand the CINⅢ or the squamous cervical cancer was significant differences.The incidence of HPV integrated and amplification of hTERC were amplification,the same two ways to predict the effect of cervical cancer.Conclusions：The strongly associated events of Genomic integration of oncogenic HPV and gain of the human telomerase gene TERC in the progression of uterine cervical dysplasia to invasive cancer.HPV infection is earlier than HPV integration and TERC gain,but the timing and relevance of HPV integration and TERC gain is not clear.