毛细管电泳法检测牛奶中硫氰酸钠质量浓度

Determination of sodium thiocyanate in milk by CE

运用毛细管区带电泳法直接检测牛奶中硫氰酸钠质量浓度。前处理使用乙睛沉淀蛋白，中性氧化铝sPE柱除去部分水溶性维生素、氨基酸，离心脱脂。Nail2PO4-Na2840，缓冲液中加入溴化十四烷基三甲基溴化铵（TTAB）作为电渗流反相作用剂，分离电压20kV，分离温度30℃，电极反向，以二极管W3r,l紫外检测N218nm波长下分析样品，质量浓度范围5-500mg／L内线性较好，r〉0．9995。该方法可适用于生鲜乳和巴氏灭菌成品乳，检测限可达到O．2mg／kg，定量范No．5-50mg／kg，精确度〈10％，回收率〉90％。

A method to determinate sodium thiocyanate in milk by CE was established. The separation was performed on NaH2PO4-YaeB407 with TTAB used as the electro-osmotic flow （EOF） modifier to change the direction of EOF. The detection wavelength was 218 nm. The separate votege was 20 kV and the separate temperature was 30 ℃. Good linearity was showed within the range of 5-500 mg/L respectively, r 〉 0.9995. The average recoveries was larger than 90.0% respectively, RSD〈IO%. The method was simple, rapid and reliable for the simtflta- neous determination of sodium thiocyanate in milk with the LOD of 0.2 mg/kg and the determination range of 0.5-50 mg/kg.