高效液相色谱法快速测定乳粉中叶黄素的质量分数

Determination of lutein in milk powder by HPLC

建立一种测定配方奶粉中叶黄素质量分数的高效液相色谱方法。采用Diamonsil C18色谱柱(5μm,250 mm×4.6 mm),流动相为甲醇-水(97∶3),流速1.0 mL/min,检测波长445 nm,采用空白基质加标实验绘制标准曲线,该方法在0.5～100 mg/L的质量浓度范围内线性关系良好(R=0.9987),加标水平为0.50,1.00,5.00μg/g时,回收率分别介于96.8%～98.4%之间,相对标准偏差介于4.27%～6.73%之间,该方法检测限低于0.10μg/g。该方法操作简便,灵敏度高、准确度高、重现性好,适合于配方奶粉中叶黄素的测定。

A HPLC method for determination of lutein in milk powder. The analysis was carried on a Diamonsil C18 （5 Ixm,250 minx4.6 mm）columu, the mobile phase was methanol-water （97：3）,the flow-rate was set at 1.0 mL. min 1, chromatographic runs were montitored at 445 nm. When analyzing samples under the same conditions, draw matrix calibration curves.The results showed that the hnearity of the method was good between 0.5-100 mg/L （R=0.9987）. The recoveries were in the range from 96.8% to 98.4% at the spiked levels of 0.50,1.00,5.00 μg/g, and the relative standard deviation were between 4.27%-6.73% in different concentration level.The limit of detection was 0.10 μg/g.The method is simple, sensitive, accurate and precise for determination oflutein in milk powder.