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前扣带皮层中碱性成纤维细胞生长因子及其受体在慢性炎症性疼痛中的表达变化

Expression of basic fibroblast growth factor-2 and its receptors in the anterior cingulate cortex of the rat with chronic inflammatory pain
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摘要 目的探讨完全弗氏佐剂(CFA)外周注射诱导的碱性成纤维细胞生长因子(FGF-2)及其受体在大脑前扣带皮层(ACC)中的表达变化。方法单侧足底注射CFA 150μl诱导大鼠慢性炎症性疼痛,在不同时间点取前扣带皮层(每组3只动物),通过RT-PCR和Western blotting方法分别检测FGF-2及其主要受体FGFR1-4mRNA和FGF-2蛋白的表达变化。结果大鼠单侧足底皮下注射CFA诱导了慢性炎症性疼痛。注射对侧脑区中,在注射后6 h、3 d、7 d、14 d,ACC中FGF-2和FGFR1的mRNA表达相对于正常组明显增加,FGFR2 mRNA在3 d、7 d、14 d表达增加。注射同侧脑区中,FGF-2和FGFR1mRNA的表达在注射CFA后6 h、3 d、7 d、14 d相对于正常组明显增加。双侧脑区的FGF-2蛋白表达在注射CFA后6 h、3 d和7 d明显增加。结论 CFA诱导的慢性炎症性疼痛状态下,FGF-2可能通过受体FGFR1参与疼痛在高级中枢部位的信号调节。 Objective To observe the expression of basic fibroblast growth factor-2 （FGF-2） and its receptors in the anterior cingulate cortex （ACC） of the rat with chronic inflammatory pain. Methods Complete Freund＇ s adjuvant （CFA） was injected into the rat＇s unilateral hind paw to induce chronic inflammatory pain. The bilateral ACC was harvested at 4 different time points after CFA injection （n = 3, for each time point）. RT-PCR and Western blotting were used to examine the mRNA and protein expression of FGF-2 and its receptors, respectively. Results Unilateral paw injection of CFA induced pain hypersensitivity. After CFA injection, the expression of FGF-2 and FGFR1 mRNA increased at the 6th hour, 3rd day, 7th day, 14th day in the ipsilateral and contralateral ACC. FGFR2 mRNA increased at the 3rd day, 7th day and 14th day in the ipsilateral ACC. The FGF-2 protein also increased in bilateral ACC at the 6th hour, 3rd day and 7th day. Conclusion FGF-2 may be involved in the processing of painthrough FGFR1 in the ACC under chronic inflammatory pain condition.
作者 钱丽玲 张志军 朱俐 高永静
机构地区 南通大学航海医学研究所
出处 《解剖学报》 CAS CSCD 北大核心 2012年第2期161-165,共5页 Acta Anatomica Sinica
基金 国家自然科学基金资助项目(30500153) 江苏省高校自然科学研究计划资助项目(05KJB180100) 江苏省自然科学基金资助项目(BK2010273) 江苏省高校优势学科建设工程资助项目
关键词 完全弗氏佐剂 碱性成纤维细胞生长因子 炎症性疼痛 前扣带皮层 反转录-聚合酶链式反应 免 疫印迹法 大鼠 Complete Freund＇s adjuvant Basic fibroblast growth factor Inflammatory pain Anterior cingulate cortex RT-PCR Western blotting Rat
分类号 R338.2 [医药卫生—人体生理学]
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参考文献19

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二级参考文献11

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解剖学报
2012年 第2期

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