骨桥蛋白对小鼠体外受精及早期胚胎发育的影响

Effects of osteopontin on in vitro fertilization and embryo development of mice

目的探讨骨桥蛋白(OPN)对小鼠体外受精及早期胚胎发育的影响。方法 120只昆明雌鼠和30只雄鼠,采用体外受精、胚胎培养方法,将小鼠精子、卵子、原核期胚胎和2-细胞期胚胎分别用不同浓度的OPN抗体预处理,观察小鼠受精、卵裂以及早期胚胎发育情况。结果不同浓度的OPN抗体分别预处理精子和卵子后,与对照组比较,受精率显著降低(P<0.01)。OPN抗体预处理原核期胚胎后,0.01mg/L OPN抗体组的卵裂率较对照组低,但差异无显著性(P=0.052),1.00mg/L OPN抗体组的卵裂率低于0.01mg/L组(P<0.01)及0.10mg/L组(P<0.05)。不同浓度的OPN抗体预处理2-细胞胚胎后可抑制胚胎发育。1.00mg/L OPN抗体组的4-细胞率和8-细胞率与0.10mg/L OPN抗体组相比差异无显著性(P>0.05),但前者的囊胚形成率显著低于后者(P<0.01)。1.00mg/L OPN抗体组的4-细胞率、8-细胞率以及囊胚形成率均显著低于0.01mg/L OPN抗体组(P<0.01)。结论 OPN可促进小鼠的受精和早期胚胎发育。

Objective To investigate the effect of osteopontin （OPN） on in vitro fertilization and embryo development of the mouse. Methods Totally 120 females and 30 males of Kunming white strains mice were used in our experiment. Mouse sperms, ova, pronucleus embryos, and 2-cell embryos were treated with various concentrations of OPN antibody （OPN-Ab）. Fertilization, cleavage and embryo development were observed. Results When mouse sperms and ova were pretreated by various concentrations of OPN-Ab,the fertilization rate was significantly lower than that of the control group （P 〈 0. 01 ）. When pronucleus embryos were pretreated by various concentrations of OPN-Ab, there were no significant differences in the cleavage rate between the 0. 01mg/L OPN-Ab and the control group （P = 0. 052）. The cleavage rate in the 1.00mg/L OPN-Ab group was significantly lower than those in 0. 01 mg/L （P 〈 0. 01 ） and 0.10mg/L OPN-Ab group （ P 〈 0. 05 ）. Embryonic development was inhibited when 2-cell embryos were pretreated by various concentrations of OPN-Ab. There were no significant differences in both the 4-cell and the 8-cell cleavage rates between in the 1.00mg/L OPN-Ab group and in the 0. 10 mg/L OPN-Ab group （P 〉 0. 05 ） ,but their blastocyst formation rates were lower in the 1. 00mg/L OPN-Ab than those in the 0. 10mg/L OPN-Ab group （P 〈 0. 01 ）. The blastoeyst formation rates of both 4-cell and 8-cell groups were significantly lower in the 1.00mg/L OPN-Ab group than those in the 0. 01 mg/L OPN-Ab group （P 〈 0.01 ）. Conclusion OPN may improve mouse fertilization,cleavage and early embryonic development in vitro.