远端缺血预处理对大鼠小体积肝移植术后早期再生的影响

Effects of remote ischemic preconditioning on early liver regeneration after small-for-size liver transplantation in rats

目的探讨远端缺血预处理(RIPC)对大鼠小体积肝移植后肝脏早期再生的影响。方法建立大鼠小体积肝移植模型后,48只SD大鼠随机均分为RIPC(A)组和对照(B)组,分别于移植术后2、6、12、24h检测血清ALT水平,并采集肝脏组织标本以观察形态学变化。采用免疫组织化学法检测Ki-67表达来评价肝脏细胞增殖情况,RT-PCR和Western blot法分别检测各时间点周期蛋白D1(cyclin D1)mRNA和蛋白表达。结果与B组相比,A组各时间点血清ALT水平降低,大鼠肝脏病理损伤减轻,Ki-67阳性细胞数、cyclin D1mRNA和蛋白表达水平均明显增加(P<0.05)。结论 RIPC可能通过促进肝脏移植后肝细胞早期再生,从而减轻大鼠小体积移植术后早期肝脏损伤。

Objective To investigate the effects of remote ischemic preconditioning（RIPC） on the early liver regeneration after small-for-size liver transplantation in rats. Methods After small-for-size liver transplantation model was established,48 rats were equally randomized into 2 groups of A（treated with RIPC before liver transplatation） and B （liver transplatation without RIPC）.Serum ALT was detected at 2,6,12,24 hours after operation.The morphological changes of liver tissues were observed and the Ki-67 expression was measured by immunohistochemistry for evaluating the cell proliferation.The expressions of cyclin D1 mRNA and protein were detected by RT-PCR and Western blot,respectively. Results Compared with group B,serum ALT was decreased,the pathological injury of the liver was less,the positive expression of Ki-67 and the expressions of cyclin D1 mRNA and protein were all increased in group A（P0.05）. Conclusion RIPC can attenuate early liver injury probably via improving early hepatocyte regeneration after small-for-size liver transplantation in rats.