摘要
目的研究美洛昔康对大鼠肝缺血再灌注损伤的保护作用以及环氧化酶-2(COX-2)的表达。方法雄性SD大鼠30只,体质量(300±20g),随机分为A组(美洛昔康组)、B组(缺血再灌注组)、C组(手术对照组),其中A、B组建立70%肝缺血再灌注损伤模型。A组于缺血前30min经腹腔注射美洛昔康(3mg/kg);B组于缺血前30 min经静脉注射等量生理盐水;C组仅作麻醉、开腹、不阻断血流。术后6h,分别采用采用全自动生化分析仪、HE染色、westernblot法、生物酶法分别测定血肝功能酶(ALT,AST),肝组织病理改变,肝内COX-2的表达、肝组织内超氧歧化酶活性(SOD)和丙二醛(MDA)的含量。结果 A组应用美洛昔康后血ALT、AST以及肝组织SOD、MDA显著低于B组(P<0.01),肝脏的明显降低,肝组织形态学无显著改变。结论美洛昔康能抑制肝脏内COX-2表达,缓解肝功能下降,保护大鼠肝脏缺血再灌注损伤。
To study the effect of meloxicam on hepatic ischemia/reperfusion injury and the expression of the cyclooxygen-2 in rats. Methods Thirty nomoral male SpragueDawley rats were randomly divided into three groups, namely group A that was subjected to ischemia/reperfusion injury with meloxicam （3mg/kg） 30 min before ischemia; group B with the same injury followed by saline administration in the same manner ,and group C with only anesthetization leparotomy but not ischemia. Animal were killed at 6 hours after reperfusion. Routine assays were performed for testing the levels of alanine aminotransferase （ALT）, aspartate aminotransferase（AST）, lthe activity of hepatic SOD, MDA and the expression of the cyclooxygen-2. The pathological changes in the liver were evaluated in hematoxylin and eosin （HE） stained sections. Results The levels of serum ALT, AST and the activity of SOD, MDA in hepatic tissues increased after hepatic ischemiaJreperfusion injury were also increased significantly. But these effects were offset by administration of meloxicam （P〈 0.01）. In group B, widespread pathological changes was observed in the hepatic tissue following hepatic ischemia/ reperfusion injury, while similar changes were scarcely visible in groupe A duo to the protective effect of intraperitoneal administration of meloxicam 30 min before ischemia. Conclusion The meloxicam can depress the expression of the cyclooxygen-2 and alleviat the Liver function during the ischemia and reperfusion period and protect the liver from ischemia and reperfusion injury.
出处
《中国医药指南》
2012年第7期3-5,共3页
Guide of China Medicine
