摘要
目的建立一种实时荧光RT-PCR方法,定量检测中耳胆脂瘤组织中TLR4、MyD88的表达水平。方法选取中耳胆脂瘤组织25份为对照组(其中13份为炎症组,12份为非炎症组);10份正常外耳道皮肤作为正常组。采用实时荧光RT-PCR与Lightcyeler荧光PCR仪定量检测TLR4、MyD88 mRNA的表达水平;采用2-ΔΔCT法处理实时荧光定量RT-PCR数据。结果炎症组TLR4、MyD88mRNA表达水平较正常外耳道组分别上升了4倍和3倍,且差异有统计学意义(P值均<0.05);炎症组TLR4、MyD88mRNA表达水平较非炎症组分别上升了2倍和1倍,且差异有统计学意义(P值均<0.05)。结论中耳胆脂瘤组织中,尤其是炎症反应明显的胆脂瘤组织,TLR4及其下游信号转导分子的表达量发生改变,提示TLR4-MyD88信号传导途径可能参与了胆脂瘤型中耳炎的发病。
To establish a real-time fluorescence quantitativepolymerase chain reaction （PCR） method to detect the expression of TLR4, MyD88 mRNA in human cholesteatoma tissues. Method Twentyfive cholesteatoma samples（Inflammation in 13 patients of which, 12 cases of noninfiammato） and ten normal auditory canal skin specimens were emollcd in this study, and expressions of TLR4 and MyD88 were determined by reverse transcriptasepolymerase chain reaction（RT-PCR） and the data were analyzed by the method of 2AACT. Result Inflammation group TLR4, MyD88mRNA canal group than the normal expression levels Astatistically significant （P〈 0.05）; inflammation group TLR4, MyD88mRNA expression levels compared with noninflammatory group were increased 2 times and 1 times, and the difference was statistically significant （P〈 0.05）. Conclusion The middle ear cholesteatoma, especially obvious cholesteatoma inflammation, TLR4 and its downstream signal transduction molecules expression changes, suggesting that TLR4-MyD88 signaling pathway may be involved in cholesteatoma of the disease.
出处
《中国医药指南》
2012年第7期26-28,共3页
Guide of China Medicine
