趋化因子IL-8和受体CXCR1,CXCR2在胃肠和肝肿瘤中的表达

Expression of IL-8,CXCR1 and CXCR2 in gastrointestinal and hepatic cancers

为研究趋化因子IL-8和受体CXCR1、CXCR2在胃肠和肝肿瘤中的作用,应用免疫组织化学和RT-PCR的方法检测了趋化因子IL-8和受体CXCR1、CXCR2在食道癌、胃癌、结直肠癌和肝癌中的表达。在胃肠肿瘤中,CXCR1在癌细胞的细胞核膜和细胞质中表达,CXCR2在癌细胞的细胞质中表达。在肝癌中,CXCR1和CXCR2在癌巢中有表达而在邻近的肝组织中无表达。应用RT-PCR的方法检测了受体CXCR1、CXCR2的转录,其结果证实了免疫组织化学的结果。在胃肠和肝肿瘤中检测到了IL-8的表达。进一步分析表明在胃肠和肝肿瘤中IL-8与CXCR1/CXCR2共表达(p=0.0448)。实验结果说明趋化因子IL-8和受体CXCR1、CXCR2可能在胃肠和肝肿瘤的发生和发展中起作用。

To study the role of IL-8 and its receptors CXCR1 and CXCR2 in gastrointestinal and hepatic cancers, ex- pression of IL-8 and its receptors CXCRI and CXCR2 were investigated in esophageal, gastric, colorectal cancer as well as hepatic cancer by using immunohistochemistry and RT-PCR assay. The positive staining of CXCR1 appeared in the nuclear membrane of malignant crypts and in the cytoplasm of malignant crypts, while CXCR2 was observed in cyto- plasm of the tumor nest of gastrointestinal cancers. In primary hepatic cancer, CXCR1 and CXCR2 were stained in the cytoplasm of the tumor nest, but not in the adjacent hepatic tissue. Furthermore, expression of IL-8, CXCR1 and CX- CR2 transcripts was analyzed by RT-PCR assay. CXCR1 and CXCR2 transcripts were detected, which confirmed the results of immunohistochemistry. IL-8 transcripts were detectable in primary gastrointestinal and hepatic cancers, sug- gesting that IL-8 involved in gastrointestinal and hepatic cancers. Further analysis showed that there was consistency of the presence of IL-8 and CXCR1/CXCR2 in primary gastrointestinal and hepatic cancers （p = 0. 0448 ）. These data sug- gest that IL-8 together with its receptors CXCR1 and CXCR2 may play roles in promoting gastrointestinal and hepatic tumor development and progression.