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原发性肝癌血管内皮细胞的分离及肝再生磷酸酶-3(PRL-3)的表达 被引量：1

Isolation of endothelial cells from hepatocellular carcinoma and their PRL-3 expression
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摘要 目的本研究通过分离纯化肝癌血管内皮细胞,检测在肝癌血管内皮细胞中肝再生磷酸酶3(phosphataseof regenerating liver 3,PRL-3)的表达,以探讨PRL-3与肿瘤血管生成和侵袭的关系。方法收集山东大学附属省立医院肝胆外科行手术切除的肝癌组织和癌旁组织,采用CD31单抗标记的免疫磁珠分离并纯化血管内皮细胞。检测血管内皮细胞中PRL-3、基质金属酶蛋白酶(matrix metalloproteinases,MMP)MMP-2和MMP-9的表达。结果分选所获得的肝癌血管内皮细胞的纯度在90%以上。PRL-3在肝癌血管内皮细胞mRNA水平(PRL-3 vsβ-actin:11.50±2.95 vs 1.76±1.04,P<0.001)和蛋白水平的表达均明显升高。结论 PRL-3在肝癌血管生成和侵袭中起重要作用,有望成为新的肿瘤治疗靶点。 Objective To investigate PRL-3 expression in endothelial cells isolated from hepatocellular carcinoma（HCC） and its correlation with angiogenesis.Methods Endothelial cells were isolated from freshly collected liver tissues of 30 HCC cases using anti-CD31 conjugated magnetic microbeads.PRL-3 expression in the isolated cells was examined by real-time PCR and Western blotting.Transcript abundances of MMP-2 and MMP-9 in endothelial cells were investigated by real-time PCR.Results The isolated endothelial cells exhibited a typical ＂cobblestone＂ appearance.Immunostaining for the von Willebrand factor proved positive in more than 90% of the isolated cells,with their PRL-3 expression being significantly up-regulated（PRL-3 vs β-actin： 11.50±2.95 vs 1.76±1.04,P0.001）.Conclusion The results strongly suggested that endothelial cells＇ PRL-3 is involved in the angiogenesis of HCC and,hence,could be a useful marker for anti-angiogenesis therapy.
作者 赵文博 刘新 刘小倩 崔新 王欣
机构地区 山东大学附属省立医院血液科 青岛大学医学院附属烟台毓璜顶医院血液科
出处 《山东大学学报（医学版）》 CAS 北大核心 2012年第3期108-112,共5页 Journal of Shandong University:Health Sciences
基金 山东省科技攻关项目(2006GG2202007)
关键词 癌 肝细胞 内皮细胞 肝再生磷酸酶-3 血管生成 Hepatocellular carcinoma Endothelium Phosphatase of regenerating liver 3 Angiogenesis
分类号 R735.7 [医药卫生—肿瘤]
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参考文献21

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同被引文献37

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山东大学学报（医学版）
2012年 第3期

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