曲安奈德对PVR中增殖细胞抑制效果的观察

Inhibition effect of triamcinolone acetonide on proliferative cells in PVR

目的:探讨不同浓度曲安奈德对体外培养的视网膜前膜细胞、视网膜色素上皮细胞(RPE)及神经胶质细胞的抑制率差异,找出视网膜前膜细胞达最大抑制率时的最低有效药物浓度,用于指导临床外伤性增生性玻璃体视网膜病(PVR)防治。方法:用四唑盐(MTT)比色法检测不同浓度曲安奈德(TA)对体外培养的视网膜前膜细胞、RPE及神经胶质细胞的作用。结果:三种细胞对TA的反应不完全相同,其中视网膜前面细胞达最大抑制率时所需要TA的最低有效药物浓度0.8mg/mL,RPE细胞最大抑制率所需TA最低有效药物为0.4mg/mL,神经胶质细胞最大抑制率所需TA最低有效药物为0.6mg/mL;且抑制率与药物作用时间呈正相关性。结论:TA能够有效抑制体外培养的视网膜前膜细胞、RPE细胞及神经胶质细胞的增生;抑制视网膜前膜细胞增殖所需TA药物浓度明显高于RPE和神经胶质细胞。

AIM：To compare the inhibition ratio of different concentration triamcinolone acetonide（TA）on retinal pigment epithelium（RPE） cells,retinal glial（RG） cells and epiretinal membrane（ERM） cells,and find the lowest effective concentration for ERMs cells up to maximum inhibition rate to guide clinical PVR prevention METHODS：Effect of TA with different concentraion（0.8,0.6,0.4,0.3,0.2,and 0.1mg/mL） on RPE cells,RG cells,and ERMs cells cultured in vitro was detected by methyl thiazolyl tetrazolium（MTT） assay.RESULTS：Three kinds of cells needed TA with different concentraion when they got the maximum inhibition ratio.The lowest effective concentration for RPE cell was 0.4mg/mL,that for ERMs cell was 0.8mg/mL and for RG cell was 0.6mg/mL.Inhibition ratio and time of drug action were positively related.CONCLUSION：TA can inhibit all kinds of cellular proliferation;but the concentration is different for three kinds of cells.The ERMs cells need higher concentration than RPE and RG cells.