依普拉芬在牵张成骨中的作用

Influence of Ipriflavone on distraction osteogenesis

背景:有研究表明适宜浓度的依普拉芬可以促进鸡胚额骨成骨细胞增殖与分化,提高其碱性磷酸酶活性,增强成骨细胞矿化能力,促进骨形成。目的:观察依普拉芬在用种植型牵张器对兔下颌牵张时成骨的影响。方法:选择日本大耳白兔建立下颌骨牵张成骨动物模型,再用依普拉芬干预。于牵张后1d,1,2,4周取材,进行血清学检查,并采用免疫组织化学方法观察转化生长因子β1在不同时间段的表达情况。结果与结论:依普拉芬干预的模型兔在牵张后1d,1,2,4周的骨小梁逐渐形成,成骨细胞逐渐增多,血清碱性磷酸酶活性和转化生长因子β1的表达均增高(P<0.05)。结果证实依普拉芬在牵张成骨中可有效加速新骨的形成。

BACKGROUND：The reports have shown that suitable concentration of Ipriflavone can promote the proliferation and differentiation of frontal osteoblasts of chick embryo,improve the alkaline phosphatase activity,increase the mineralization capacity of osteoblast and promote bone formation.OBJECTIVE：To observe the effect of Ipriflavone on distraction osteogenesis of rabbits through implanted-distraction device.METHODS：Forty Japanese white rabbits were used to establish the mandibular distraction osteogenesis models,and induced with Ipriflavone.At 1 day,1,2 and 3 weeks after distraction,the serological examination was preformed,and the expression of transforming growth factor-β1 at different time points was observed by immunohistochemistry.RESULTS AND CONCLUSION：At 1 day,1,2 and 4 weeks after distraction,there was trabecular bone in the model induced with Ipriflavone,the osteoblasts were increased,and the alkaline phosphatase activity and transforming growth factor-β1 expression was increased（P 0.05）.Ipriflavone can effectively accelerate bone formation in distraction osteogenesis.