基质金属蛋白酶7和转化生长因子β1在病理性瘢痕中的表达:与正常瘢痕及正常皮肤组织比较

Expressions of matrix metalloproteinase 7 and transforming growth factor beta-1 in pathological scars compared with normal scars and healthy skin tissues

背景:从基因和蛋白角度深入探讨病理性瘢痕的发病机制具有重要价值。目的:检测病理性瘢痕中基质金属蛋白酶7和转化生长因子β1蛋白的表达。方法:选择2004/2009年唐山市工人医院烧伤整形科手术后保存的病理性瘢痕标本,其中瘢痕疙瘩54例,增生性瘢痕42例。选取同期45例因非感染性疾病行手术切除的正常瘢痕组织作为对照组,选取同期45例正常皮肤组织作为正常对照组。采用流式细胞术半定量检测组织中基质金属蛋白酶7和转化生长因子β1的表达。结果与结论:基质金属蛋白酶7和转化生长因子β1在病理性瘢痕中的表达量明显高于正常瘢痕组织和正常皮肤组织,基质金属蛋白酶7和转化生长因子β1在正常瘢痕组织中的表达明显高于正常皮肤组织,病理性瘢痕和正常瘢痕中基质金属蛋白酶7和转化生长因子β1的表达呈正相关。结果表明基质金属蛋白酶7和转化生长因子β1的高表达及协同作用,可能促进病理性瘢痕的发生发展。

BACKGROUND：A thorough study on pathological scar pathogenesis at gene and protein level is of great value.OBJECTIVE：To explore the expressions of matrix metalloproteinase 7 and transforming growth factor β1.METHODS：Pathological scar samples were collected after surgeries carried out in the Department of Burn and Plastic Surgery,Workers＇ Hospital of Tangshan,between 2004 and 2009.There were 54 samples of keloid and 42 samples of hypertrophic scar.A total of 45 regular scar samples gained from surgical resection due to noninfectious diseases were selected as control group.The expressions of matrix metalloproteinase 7 and transforming growth factor β1 were semi-quantitatively examined by flow cytometry.RESULTS AND CONCLUSION：The expression of matrix metalloproteinase 7 and transforming growth factor β1 in pathological scars were significantly higher than that in the normal scars and healthy skin tissues.The expressions of matrix metalloproteinase 7 and transforming growth factor β1 in normal scars were significantly higher than those in the healthy skin tissues.The expressions of matrix metalloproteinase 7 in pathological scars and normal scars were positively correlated with the expression of transforming growth factor β1.These results indicate that the high level expression and synergistic effect of matrix metalloproteinase 7 and transforming growth factor β1 may promote the occurrence and development of pathological scars.