黄芪多糖胶原干预周围组织中核因子κB与血管内皮细胞生长因子表达而促进毛细血管新生

Enhancing angiogenesis by improving the expression of vascular endothelial growth factor and nuclear factor kappa B in surrounding tissues induced by astragalus polysaccharides collagen

背景:补气生血类中药具有与生长因子促血管生成作用相近的功效。目的:观察黄芪多糖胶原促血管新生的疗效,及其对血管内皮细胞生长因子基因表达的核因子κB通路的影响。方法:采用大鼠背部皮下植入模型,分别将黄芪多糖胶原和空白胶原植入大鼠背部两侧,于植入后3,7,14,21d处死大鼠,取胶原周围肉芽组织进行检测。结果与结论:造模后3d时,大鼠肉芽组织中微血管数开始增加,7～14d时达到峰值,之后下降。在造模后3,7,14d,植入黄芪多糖胶原的大鼠周围肉芽组织中毛细血管数、血管内皮细胞生长因子mRNA及核因子κB蛋白的表达均显著高于植入空白胶原的大鼠(P<0.01),且核因子κB蛋白的表达早于毛细血管新生与血管内皮细胞生长因子mRNA的表达。说明黄芪多糖胶原促血管新生作用有可能是通过活化核因子κB信号通路而上调血管内皮细胞生长因子mRNA表达实现的。

BACKGROUND：Lots of traditional Chinese medicines with the effects of nourishment and angiogenesis have the similar functions with those of angiogenic growth factors.OBJECTIVE：To explore the angiogenic mechanism of astragalus polysaccharides（APS） collagen sponge by its regulating expression of vascular endothelial growth factor（VEGF） mRNA and the up signal pathway of nuclear factor κB（NF-κB） protein.METHODS：Collagen sponges with and without APS immobilization were implanted inside subcutaneous pockets for 3,7,14 and 21 days respectively.The surrounding granulation tissue of collagen was taken to detect.RESULTS AND CONCLUSION：The number of miocrovessels in granulation tissue was increased at 3 days after modeling and reached to peak at 7 to 14 day,and then decreased gradually.The number of miocrovessels in granulation tissue and the expressions of VEGF-mRNA and NF-κB protein of surrounding tissues of collagen sponges with APS immobilization were higher than that without APS immobilization（P 0.01）.The protein expression of NF-κB was earlier than the expression of VEGF-mRNA and newborn miocrovessels.The partly angiogenic mechanisms of collagen sponges with APS may be realized through the activation of the NF-κB protein pathway to improve VEGF-mRNA expression in the surrounding tissues.