聚焦超声对羊肝脾组织的损伤效应

Damage effect of focused ultrasound on sheep liver and spleen

背景:高强度聚焦超声可引起组织损伤。目的:研究聚焦超声对羊肝脏和羊脾脏组织的损伤效应。方法:用不同强度和不同方法的聚焦超声波辐照5只羊的体内肝脏、体外肝脏及体外脾脏,观察其辐照后大体及病理学改变。结果与结论:聚焦超声辐照体外羊肝的即刻组织损伤反应较辐射治疗1周后的体内羊肝重;体内羊肝及体外羊肝在不同治疗强度下"#"法的组织损伤反应较"="法重;不同强度及不同方法辐照体外羊脾后均未见明显组织反应;聚焦超声Ⅲ档"#"法辐照羊肝组织可见多个灶性的肝细胞变性及凝固性坏死;聚焦超声Ⅳ档"#"法辐照体内羊肝组织可见被膜下炎性纤维肉芽肿性包裹结节,中心大片凝固性坏死;聚焦超声Ⅳ档"#"法辐照体外羊肝组织可见被膜下大部分区域肝小叶结构零散不清,肝窦明显扩大,肝细胞索明显变窄,凌乱不清。说明聚焦超声Ⅲ档"#"法能够按照设计要求选择性的损伤羊肝脏组织。

BACKGROUND：High-intensity focused ultrasound can induce tissue damages.OBJECTIVE：To study the damage effect of focused ultrasound on the tissues of sheep liver and spleen.METHODS：Focused ultrasounds with different intensities and different methods were used to radiate the livers in vivo,livers and spleens in vitro of five sheep.Visual and pathological changes were observed after radiation.RESULTS AND CONCLUSION：The immediate damage of tissues of livers in vitro with focused ultrasound was more severe than the livers in vivo after 1 week of radiation therapy;in therapies with different intensities,the tissue damage of ＂#＂ method was more severe than ＂=＂ method in both in vivo and in vitro sheep livers;no significant damage was observed in sheep spleens in vitro with radiation therapies with different intensities and methods;focused ultrasound radiation of III file and ＂#＂ method resulted in a great number of hepatic cells with focal degeneration and coagulative necrosis;focused ultrasound radiation of IV file and ＂#＂ method on in vivo sheep liver tissues resulted in inflammatory fibrous granulomatous nodules under envelope and coagulative necrosis in large central areas;focused ultrasound radiation of IV file and ＂#＂ method on sheep liver tissues in vitro resulted in scattered hepatic lobule structures in most regions under envelope,significantly expanded hepatic sinus and significantly narrower hepatic cords,messy and unclear.Focused ultrasound with III file and ＂#＂ methods can damage sheep hepatic cells selectively according to design requirements,and provided experimental basis of treatment of allergic rhinitis.