实时PCR法验证心肌梗死大鼠模型正常组织及心肌梗死组织与血管新生途径相关差异基因的表达

Verification of differential expression of angiogenesis related genes in normal tissues and myocardial infarction tissues of rat models of myocardial infarction using real-time PCR method

背景:研究表明急性心肌梗死后血管新生过程受众多基因调控。目的:观察急性心肌梗死后与血管新生途径相关的差异基因的表达。方法:建立急性大鼠心肌梗死模型,在基因芯片结果基础上选取5个特定的表达差异基因:分泌磷酸蛋白1、趋化因子受体2、人血管生成素样蛋白4、CXC趋化因子配体5和白细胞介素1β。采用实时PCR法验证5个基因在心肌梗死大鼠模型正常组织及心肌梗死组织中的表达。结果与结论:分泌磷酸蛋白1、趋化因子受体2和人血管生成素样蛋白4在心肌梗死急性期表达上升显著(P<0.05),呈时间依赖性。CXC趋化因子配体5和白细胞介素1β基因表达水平呈低位波动,变化不显著。其中分泌磷酸蛋白1与趋化因子受体2与急性心肌梗死后炎性、细胞黏附、迁移和趋化相关,而人血管生成素样蛋白4与血管新生和细胞分化相关。

BACKGROUND：Researches have shown that the process of angiogenesis after acute myocardial infarction is mediated by a number of genes.OBJECTIVE：To investigate the differential expression of angiogenesis related genes after acute myocardial infarction.METHODS：Rat model of acute myocardial infarction was constructed;five specific differential expression genes in gene chip were selected based on the results of gene chips：secreted phosphoprotein 1,chemokine receptor 2,angiopoietin-like 4,CXC chemokine ligand 5 and interleukin-1β.Real-time PCR was conducted to detect the expression levels of these genes in normal and in infarction myocardial tissues of rat acute myocardial infarction model.RESULTS AND CONCLUSION：The gene expression of the secreted phosphoprotein 1,chemokine receptor 2 and angiopoietin-like 4 increased significantly in the early stage of acute myocardial infarction（P 0.05）;and it was time-dependent.While the expression levels of CXC chemokine ligand 5 and interleukin-1β showed low fluctuation without significant changes.Among them,the secreted phosphoprotein 1 and chemokine receptor 2 are related to the inflammation,cell adhesion,migration and chemotaxis after acute myocardial infarction;while the angiopoietin-like 4 is related to angiogenesis and cell differentiation.