高同型半胱氨酸血症及其所致动脉粥样硬化动物模型的复制

Copying of arteriosclerotic animal models induced by hyperhomocysteinemia

背景:高同型半胱氨酸是动脉粥样硬化的危险因素之一,而血管拉伤后的新生内膜增生可能有助于动脉粥样硬化的形成。目的:研究采用高蛋氨酸饲料喂养结合球囊损伤腹主动脉的方法复制兔高同型半胱氨酸血症及其所致动脉粥样硬化模型的可行性。方法:将32只白兔随机分成4组:分别给予腹主动脉内膜球囊损伤和(或)2%L-蛋氨酸颗粒饲料,以正常饲养的兔作为对照。分别于实验的第0,2,12周采血化验,并于实验的第12周处死白兔取腹主动脉标本观察。结果与结论:给予2%L-蛋氨酸颗粒饲养2周后,兔血清高同型半胱氨酸水平明显增高(P<0.05),此时予腹主动脉内膜球囊损伤,12周后在兔损伤血管段形成了特有的动脉粥样硬化病变,并且同时给予腹主动脉内膜球囊损伤和2%L-蛋氨酸颗粒饲料的兔粥样斑块面积更大、内膜损伤更严重。说明采用蛋氨酸饲料喂养结合球囊损伤兔腹主动脉内膜的方法可在短时间内建立高同型半胱氨酸血症及动脉粥样硬化模型。

BACKGROUND：Hyperhomocysteinemia is one of the risk factors for atherosclerosis,and intimal proliferation after artery injury may lead to the formation of atherosclerosis.OBJECTIVE：To research the feasibility of copying arteriosclerotic animal model induced by hyperhomocysteinemia with L-methionine to feed rabbits and balloon catheter to strain abdominal aorta into injury.METHODS：Thirty-two healthy white rabbits were divided into four groups.Abdominal aortic balloon injury and（or） 2% L-methionine pellets were given to the groups respectively,the rabbits with normal feeding were as control.Blood was drawn for laboratory test at the beginning and end of the 2nd and 12th weeks respectively.The rabbits were executed and abdominal aortas were taken out to do pathology dyeing at the end of the 12th week.RESULTS AND CONCLUSION：Homocysteine levels of model group were significantly higher than that of control group at the end of the 2nd week（P 0.05）.Diffusive confluent atheromatous plaques formed along the abdominal aorta at 12 weeks after abdominal aortic balloon injury.And the rabbits that were given the abdominal aorta balloon injury and 2% L-methionine pellets had a larger plaque and more serious intimal injury.It indicates that the arteriosclerotic animal model induced by hyperhomocysteinemia can be established in a short time by 2% L-methionine feeding and balloon injury abdominal aorta.