实验用脊髓腹侧损伤撞击器的设计及应用

Design and application of a new ventral spinal cord injury impactor

背景:目前脊髓损伤的基础研究主要依赖于动物损伤模型,每种脊髓损伤模型都有其适应范围及不足之处。目的:设计并制造实验用大鼠脊髓腹侧损伤撞击器,并在大鼠脊髓损伤实验中验证仪器的使用效果。方法:设计脊髓腹侧损伤撞击器的图纸,并按图纸组装成成品;应用该仪器制作SD大鼠脊髓腹侧损伤模型。随机分为3组,中度损伤组用23V电压的打击力量,重度损伤组用25V电压打击力量,对照组在安装撞击钩后,不实施打击。结果与结论:重度损伤组BBB评分明显低于中度损伤组(P<0.001)。BBB评分与脊髓残留组织呈线性相关(P<0.001),23V电压力量30%的脊髓变形率可以制作中度脊髓损伤模型,25V电压力量61%的脊髓变形率可以制作重度脊髓损伤模型。证实实验设计的脊髓腹侧损伤仪器能够制作大鼠脊髓腹侧中、重度损伤模型,损伤力量大小方便可控,脊髓形变可测。

BACKGROUND：At present,the research on the spinal cord injury（SCI） is mainly depend on the animal model,each kind of SCI model has its adapted area and inadequate.OBJECTIVE：To design and manufacture a ventral SCI impactor model of rats,then evaluate the performance of the device in rat SCI experiment.METHODS：The chart paper of the ventral SCI impactor was made and then the device was established according to the chart paper.The SD rat ventral SCI model was made by the device.The models were divided into three groups randomly,moderate injury group,severe injury group and control group.Moderate injury group needed a beat of 23 V,severe injury group needed a beat of 25 V,no beat in the control group.RESULTS AND CONCLUSION：The BBB score in the severe injury group was significantly lower than that in the moderate injury group（P 0.001）,BBB score was linearly related to spinal cord residual tissue（P 0.001）.23 V voltage power and 30% cord deformation rate results in the moderate injury model,25 V voltage power and 61% cord deformation rate results in the severe injury model.This ventral spinal cord injury device can reliably produce moderate and severe cord injury models,and it is used easily.The injury force is controllable and the spinal deformation is measurable.