静水压下芹菜素对体外培养人髓核细胞凋亡率及基质代谢的影响

Effect of apigenin on apoptotic rate and matrix metabolism of human nucleus pulposus cells cultured in vitro under hydrostatic pressure

背景:生物力学环境的异常及一氧化氮过度表达是椎间盘退变进程中重要的影响因素。目的:探讨静水压下芹菜素对体外单层培养的人髓核细胞凋亡率及基质代谢的影响。方法:在静水压加载系统中对体外单层培养的人髓核细胞(传4代)施以0.1～3.0MPa的静压,加压2h,同时使用芹菜素和一氧化氮供体对髓核细胞进行干预,分析不同静水压下芹菜素对髓核细胞凋亡率、基质金属蛋白酶及基质表达的影响。结果与结论:在0.3MPa静水压下,芹菜素能改善由于高浓度一氧化氮引起的髓核细胞功能抑制状态,降低基质金属蛋白酶3过表达,恢复基质金属蛋白酶3与其抑制因子1之间的平衡;而在3MPa静水压下,芹菜素能降低一氧化氮引起的髓核细胞高凋亡,并在一定程度上缓解基质金属蛋白酶3与其抑制因子1之间的不平衡。说明芹菜素能够对髓核细胞的功能状态、凋亡率,对基质金属蛋白酶3及其抑制因子1平衡产生影响,而且这种影响在不同的静水压环境下有所不同。

BACKGROUND：Abnormal biomechanical environment and over-expression nitric oxide are the two important factors in the process of intervertebral disc degeneration.OBJECTIVE：To discuss the effect of apigenin on the apoptotic rate and matrix metabolism of monolayer cultured human nucleus pulposus cells in vitro under different hydrostatic pressures.METHODS：In the hydrostatic pressure loading system,0.1-3MPa pressure was imposed on the human nucleus pulposus cells （passage 4） in monolayer culture in vitro for 2 hours,while using apigenin and nitric oxide donor to intervene on nucleus pulposus cells,and analyzing the effect of apigenin on the apoptotic rate,matrix metalloproteinase and matrix metabolism of human nucleus pulposus cells under hydrostatic pressure.RESULTS AND CONCLUSION：Under 0.3MPa hydrostatic pressure,apigenin could reduce the degradation of extracellular matrix by regulating the balance between matrix metallo proteinases-3 （MMP-3） and human tissue inhibitor of metal protease-1 （TIMP-1）,and improving nucleus pulposus cells inhibition status due to the high concentration of nitric oxide.Under 3MPa hydrostatic pressure,apigenin had a role in reducing the apoptotic rate of nucleus pulposus cells and could alleviate the unbalance between the MMP-3 and TIMP-1.The results showed that apigenin has an influence on the function status and apoptotic rate of nucleus pulposus cells,the balance between MMP-3 and TIMP-1,and the effects are different under different hydrostatic pressure environments.