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人单链白细胞介素12在酵母细胞中的表达

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摘要 IL-12是一个多功能细胞因子,由于它结构复杂只有当P_(40)和P_(35)两条链同时表达并以1:1形式构成异二聚体蛋白时才具有生物活性,亦不能在E.coli中表达。构建人单链白细胞介素12(hscIL-12)融合基因真核表达质粒进行基因转导,获得hscIL-12表达载体,pYES-hscIL-12,使hscIL-12置于半乳糖可诱导启动子GAL_1的调控之下,用醋酸锂方法将重组体转入酿酒酵母YPH500细胞。免疫印迹结果表明重组hscIL-12成功地在酵母细胞中表达,分子量约为70kD,与推测的分子量一致。
出处 《陕西医学杂志》 CAS 2000年第1期3-5,共3页 Shaanxi Medical Journal
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参考文献11

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