老年慢性肺心病患者PBMC中IL-6分泌动力学变化及临床意义

The kinetics and clinical significance of IL-6 secreted by PBMC in the elderly patients with chronic corpulmonale

目的 系统地观察老年慢性肺心病患者及正常人外周血单个核细胞 (PBMC)自分泌及体外诱生 IL 6的动力学变化与感染之间的关系。方法 采用 MTT(噻唑蓝 )比色法。结果 (1 )老年慢性肺心病患者在不同时期、时相及自分泌和体外诱导情况下 ,PBMC的 IL 6水平均明显高于正常人组 (P<0 0 1 ) ;(2 )自分泌 IL 6水平最高的是 2 4 h的急发期组 ,显示出高峰前移 ,它与本组 2 4 h的体外诱因和缓解期组的自分泌水平差异显著 (P<0 0 5) ;(3)体外诱生 IL 6水平最高的 72 h的急发期组 ,与缓解期组的 72 h自分泌和急发期组的 2 4 h诱生水平差异显著 (P<0 0 5)。结论 本实验进一步证实了 IL 6与感染密切相关 ,为 IL 6的临床应用提供实验依据 ,同时有助于预测感染的程度。

The kinetics of IL 6 autosecreted and induced in PBMC of the elderly patients with chronic corpulmorale and healthy persons were investigated systemically. The results showed that the level of IL 6 antosecreted and induced in PBMC of the patients situated in all stages of the diseases were higher than in normal control (P<0 01) . The peak of IL 6 autosecreted by PBMC was in acute stage at 24 hours and it was going formard. There was significant difference on IL 6 level whateven between autosecretion and induction at 24 hours and between in acutestage and in remisson stage (P<0 05) . The peak of IL 6 induced was in acute stage at 72 hours. There was marked difference between that induced in acute stage and both that autosecreted in remission stage at 72 hours and that induced in acute stage at 24 hours (P<0 05) . Above results indicated that there is dose. Connection between IL 6 and the inction. Which proved an experimental evidence for clinical use of IL 6.