早老性痴呆转基因小鼠的研究

Study of the Transgenic mice model of Alzheimer disease by use of Human Mutant APP gene

目的 建立早老性痴呆的转基因小鼠 ,为进一步的发病机理研究及药物筛选提供动物模型。方法 显微注射方法制备转基因小鼠 ,通过 PCR、Southern杂交鉴定。结果 质粒 p Pd AP751的 Tth1 1 1 +Xba 双酶切片段长度为 4 3kb,经回收、纯化后 ,注射到小鼠受精卵的雄性原核 ,经 PCR、Southern杂交鉴定 ,确定首建鼠一只。首建鼠现已传代建系。结论 通过显微注射的方法 ,建立了早老性痴呆的转基因动物模型。

For the etiopathological studies and drug development Alzheimer disease(AD), the transgenic mice of AD were used. Methods Obtain the transgenic mice by microinjection, and confirmed by polymerase chair reaction(PCR) and Southern blot.Results The transgene was isolated by Tth111I+XbaI from the recombinant pPDAP751, and purified by equilibrium centrifugation in CsC1 gradient. Transgene was microinjected into the male pronucleus of the zygotes. The founder was tested by PCR and Southern blot.Conclusions By microinjection, the transgenic mice of AD were establish, which carry the human mutant APP cDNA751.