云南人肉孢子虫人——牛——人间循环感染研究

STUDIES ON MAN-CATTLE-MAN INFECTION CYCLE OF SARCOCYSTIS HOMINIS IN YUNNAN

本文用云南人肉孢子虫进行了人——牛——人之间循环的实验感染研究。在光镜下观察了人粪中人肉孢子虫卵囊或孢子囊及牛肌肉中的包囊。在电镜下见到包囊表面有长达7.9μm以上密集直立指状突起,内囊壁向内形成隔膜,将包囊分隔成许多隔室,室中充满香蕉形缓殖子。包囊壁系由一层单位膜和此膜下起加固作用的一层嗜锇物质组成;紧靠内囊壁下有一条由圆形细颗粒物质组成的基质带,带内无纤丝。包囊壁规则折叠,形成栅栏状直立指状突起。在突起中有大量纤丝。在两突起之间有半泡状凹陷。并对人体实验感染肉孢子虫病的临床症状进行了描述与讨论。

The present paper reports on the results of an experimental study on man-cattle-man infection of Sarcocystis hominis, found in Yunnan Province.About ten thousand sporocysts collected from the feces of persons naturally infected with Sarcocystis hominis were fed to a calf, which was dissected 150 days later. Numerous cysts of Sarcocystis hominis were found in the cardiac and skeletal muscles. By light microscopy, the cyst wall of fresh preparation showed numerous thick, finger-like projections, with maximum length of 7.9μm(Fig,1.). By electron microscopy, the cyst had a regularly folded, with primary wall forming palisade-like protrusions. Numerous sharp invaginations found infthe protrusions were sawtooth-shaped, covering the whole surface of the protrusions (Fig. 3). No fine fibrils were observed within the zone of ground substance beneath the primary cyst wall.Two rhesus monkeys were fed with beef infected with Sarcocystis hominis and sporocysts and oocysts were found in their feces 29 and 31 days later, the patent period of sporocyst excretion bieng 5 and 7 days, respectively.The senior author had taken voluntarily 60g beef of the experimentally infected calf, and presented clinical symptoms such as anaemia, abdominal pain, diarrhoea, fatigus and dizziness on ds post infection with sporocysts and oocysts found in the feces on d,. The patent period of sporocyst excretion was more than 42 days. The mean size of 50 sporocysts was 11.90±0.04 ×15.88± 0.03μm and that of 50 oocysts, 15.56 ± 0.05 × 19.76 ±0.04μm(Fig.4). On d50 he took acetylspiramycine tablets, the initial dose being 0.4g, followed by 0.2g qid. for 15 days. 10 days after acetylspiramycine administration, the symptoms disappeared and so did the sporocysts on fecal examination.