秋水仙碱治疗家兔血吸虫病肝纤维化超微结构研究

ULTRASTRUCTURAL STUDIES ON EFFECTS OF COLCHICINE IN TREATING HEPATIC FIBROSIS OF SCHISTOSOMIASIS RABBITS

8只家兔1次感染日本血吸虫尾蚴80±5条,4个月后可导致肝纤维化。其中4只兔感染4个月后,每日服秋水仙碱40μg/kg,连服7 wk。另4只兔为正常对照。观察秋水仙碱治疗后肝细胞超微结构变化,并用图象分析仪进行形态测定胶原微纤维在狄氏腔内及肝细胞周围的分布面积。结果显示,秋水仙碱能减轻肝细胞损伤,使狄氏腔内及肝细胞周围活跃的成纤维细胞和胶原微纤维减少。感染组与治疗组胶原微纤维面积占所测量肝组织面积的百分数分别为42.5％和0.2％(P<0.91).提示秋水仙碱有抗纤维化的作用。

8 rabbits were infected percutaneously with the cercariae of Schistosoma japonicum; After hepatic fibrosis had developed in these rabbits 4 months after infection, 4 out of the 8 infected rabbits were given colchicine orally at a dosage of 40/ig/kg per day for 7 weeks. Another uninfected rabbits were used as controls. The therapeutic effects of col-chicine on hepatic fibrosis were studied by transmission electron microscopy and mor-phometry, in which the area of collagen microfibrils in the space of disse and liver cells was measured.The results showed that colchicine relieved ultrastructural injury of liver cells and reduced the number of active fibroblasts and collagen microfibrils. The area of collagen microfibrils measured in the liver of the infected rabbits and colchicine-treated rabbits accounted for 42.5% and 0.2%, respectively (P<0.01), suggesting that colchicine has therapeutic effect against schistosomal liver fibrosis (Figs. 1-4).