病患关节软骨中肥大软骨细胞的生物学特性的研究

Study on Biological Characteristics of Hypertrophic Chondrocytes of Damaged Articular Cartilage

利用原位杂交、Northernblot及免疫组化等方法检测了X型胶原、胰岛素样生长因子 (IGF -Ⅰ )及转换生长因子 (TGF - β1)mRNA在病损关节软骨 (骨关节炎、类风湿性关节炎、大骨节病和关节鼠 )及无血清培养的人的关节软骨细胞和生长板的肥大软骨细胞的表达 ,结果证明各病损的关节软骨中其细胞除簇聚的细胞外 ,单个的椭圆而大的细胞也能表达X型胶原 ,也是肥大软骨细胞 ;无血清培养的肥大软骨细胞有分化及反分化能力 ,不都是终末细胞。对肥大软骨细胞的生物学及病理生物学特性进行了讨论。

Thebiologicalandpathobiologicalcharacteristicsofthehypertrophicchondrocytes,phenotypeexpressionofα1(X)mRNA ,IGF -ⅠmRNAandTGF - β1mRNAwereexaminedbysitu -hybridization ,Northern -blotandimmunehistochemistryonthedamaged articularcartilage (OA ,RA ,Kashin -Beckdiseaseandloosebody)andthefreeserumculturedhypertrophicchondrocytesseparated fromgrowthplate Theresultsshowedthatinadditiontotheclusteredproliferatedchondrocytes,thosemono -ovallargercellsinthe damagedcartilagecanalsosecreteXcollagen ,botharehypertrophicchondrocytes Freeserumcultureofhypertrophicchondrocytes showedthattheypossestheabilityofcellsdifferentiationanddedifferentiation Itisnotakindofterminalcell