伯氏疟原虫的体外培养和扫描电镜观察

IN VITRO CULTIVATION AND SCANNING ELECTRON MICROSCOPIC OBSERVATION OF PLASMODIUM BERGHEI

采用蜡烛缸法比较大鼠血清、人脐带血清、成人B型血清、兔血清、小牛血清和加有次黄嘌呤的小牛血清对伯氏疟原虫体外生长的影响.并对培养前和培养12、20、28h的小牛血清培养物作扫描电镜观察。结果表明,所用血清均不能明显改善长期培养效果;对短期培养的效果,以大鼠血清和人脐带血清为优,次黄嘌呤对疟原虫体外生长有潜在的抑制作用。电镜观察表明,小牛血清能使大鼠红细胞发生凝集,红细胞和疟原虫表面所形成的表面粘附物能妨碍裂殖子入侵和疟原虫对代谢物的吸收和排泄。

To compare the effects of sera on the growth of Plasmodium berghei, the erythrocytic stages were cultured with rat serum, human umbilical cord serum, human B-type serum, rabbit serum, calf serum and calf serum with hypoxanthine respectively. The topography of the erythrocyte and parasite cultured with calf serum were observed before and 12, 20 and 28 hours after cultivation. All of the sera used did not effectively improve the long-term culture of P. berghei, regardless of some differences in short-term cultures. Under SEM, erythrocytes agglutinated with each other and the surface adhesive materials on the erythrocytes and parasites agglomerated gradually with the cultivation time. The results suggested that the surface adhesive materials were correlated with the destruction of erythrocytes, malnutrition of the parasites and blockade of merozoite reinvasion (Figs. 1-16).