吡喹酮治疗对实验小鼠日本血吸虫循环SJ70抗原的影响

EFFECT OF PRAZIQUANTEL TREATMENT ON THE LEVELS OF CIRCULATING SJ70 ANTIGEN IN MURINE SCHISTOSOMIASIS JAPONICA

采用双夹心酶联免疫吸附试验(双夹心ELISA)检测实验感染日本血吸虫小鼠体内循环SJ70抗原。发现感染后第4wk,即可检出此种抗原。此后,循环SJ70抗原的检出量与检出率逐周增高;至感染第7wk,全部小鼠均检出SJ70抗原,且其检出量达高峰。并持续到感染第16wk。吡喹酮治疗后,小鼠体内SJ70抗原水平逐周下降。至治疗后第5wk时,16只小鼠中14只已不再检出SJ70搞原。另2只小鼠的循环抗原水平降至近消失水平。而2只虽经吡喹酮治疗但未治愈小鼠的SJ70抗原水平无明显变化。

A circulating schistosome 70kDa antigen (SJ 70) has been detected in sera of mice infected with Schistosoma japonteum. using a double antibody sandwich enzyme-linked immunoabsorbent assay (ELISA). SJ 70 antigen was first detected in the sera of S. ja-poreicum-infected mice at the 4th week after infection and increased rapidly, reaching their peak by the 7th week after infection, and remaining relatively unchanged for additional 9 weeks. In contrast, in mice infected with S. japonicum for 7 weeks and then treated with praziquantel (100mg/kg body weight) there was a significant decrease in serum antigen levels within two weeks after treatment. An almost complete disappearance of the antigen from the sera occurred by the 5th week after treatment.The results indicated that the detection of SJ 70 antigen might be used as early diagnosis of schistosoniasis and evaluation of drug efficacy