阿司匹林对正常人表皮黑素细胞系PIG1细胞黑素生成的影响

Effects of aspirin on melanogenesis of human epidermal melanocyte cell line PIG1 cells in vitro

目的:观察阿司匹林对体外培养的正常人表皮黑素细胞系PI G1细胞的活力、黑素生成及酪氨酸酶活性的影响。方法:用不同浓度的阿司匹林处理PIG1细胞72h,CCK-8比色法测定阿司匹林对PIG1细胞活力的影响;光学显微镜观察细胞形态学变化;氢氧化钠裂解法测定黑素的含量;体外多巴氧化反应法测定酪氨酸酶活性的变化。结果:阿司匹林浓度小于或者等于500μmol/L时无明显细胞毒性;浓度大于或者等于125μmol/L时以剂量依赖方式抑制黑素生成,差异有统计学意义(P<0.05);黑素细胞酪氨酸酶活性无显著性变化(P>0.05)。结论:阿司匹林抑制PIG1细胞的黑素生成,可能应用于色素增多性皮肤病。

Objective To investigate the effects of aspirin on cell viability, tyrosinase activity and melanogenesis of human epidermal melanocyte cell line PIG1 cells in vitro. Methods After treating PIG1 cell with aspirin in different concentrations for 72h, we employed CCK-8 assay, oxidative DOPA reaction and NaOH method to measure the cell viability, tyrosinase activity and melanogenesis of PIG1 cells respectively. The morphologic changes of PIG1 cells were observed via light microscope. Results Aspirin had no significant effect on the cell viability of PIG1 cells when the concentrations were lower than 500μmol/L. The melanogenesis of PIG1 cells was remarkably decreased in a concentration-dependant manner by aspirin at the concentrations higher than 125μmol/L （P〈0.05）. However the tyrosinase activity had no significant change after aspirin treatment （P〉0.05）. Conclusion Aspirin can inhibit the melanogenesis of PIG1 cells, thus it may be developed as a clinical treatment of hyperpigmented skin diseases.