猪囊虫囊液游离氨基酸组分的研究

FREE AMINO ACID COMPONENTS OF CYSTIC FLUID OF CYSTICERCUS CELLVLOSAE

应用氨基酸自动分析仪测定猪囊虫囊液的游离氨基酸组分及含量。从14只猪的皮下肌肉囊虫及10只猪的脑囊虫的囊液进行测定。在两者囊虫囊液所测定的18种氨基酸中,均以内氨酸含量最高;在皮下肌肉囊虫囊液,其次为甘氨酸和脯氨酸,在脑囊虫囊液,则以苏氨酸为次。用配对T检验方法比较7只猪的皮下肌肉和脑囊虫囊液中各种氨基酸含量表明,在不同部位囊虫囊液所含的15种氨基酸中,有12种含量存在显著差异,特别是苏氨酸、丝氨酸、丙氨酸、锁氨酸和酪氨酸出现差异非常显著。本研究结果提示上述氨基酸代谢差异,可能与猪囊虫在宿主的寄生部位不同有关.

The free amino acids in the cystic fluid of Cysticercus cellulosae were determined quantitatively by using automatic amino acid analyzer. The cystic fluids of both subcutaneous-muscular Cysticercus cellulosae from 1.4 pigs and cerebral Cysticercus cellulosae from 10 pigs were detected. Among the 18 amino acids detected, alanine was found to be the predominant amino acid in both of the cystic fluids. The quantities of glycine and proline were higher than other amino acids in subcutaneous-muscular Cysticercus cellulosae, while threonine was higher than the rest in the cerebral Cysticercus cellulosae. A comparison was made between the quantities of amino acids in the fluids of both subcutaneous-muscular and cerebral Cysticercus cellulosae of 7 pigs by the T-test method. The results showed that the quantities of 12 out of 15 amino acids were significantly different, with particular reference to threonine, alanine, serine, and valine, suggesting that the difference in the metabolism of amino acids in Cysticercus may be related to the parasite's location in their respective hosts.