单克隆抗体Dot-ELISA检测血吸虫病循环抗原的研究.Ⅰ.

DETECTION OF CIRCULATING ANTIGEN IN SCHISTOSOMIASIS BY DOT-ELISA WITH MONOCLONAL ANTIBODY

将血吸虫肠相关阴极抗原的单克隆抗体3D8A联结过氧化物酶后用于直接法Dot-ELISA检测急性、慢性与晚期血吸虫病人血清中循环抗原,阳性率分别为90.6、83.2及30.7％。正常人血清及非寄生虫感染病人血清均未见阳性反应。EPG>100组血清阳性率及抗原水平高于EPG<100。慢性血吸虫病人经吡喹酮治疗后一年,粪检阴性者大部分血清抗原转为阴性;粪检阳性者血清检查仍为阳性。结果表明,肠相关阴极抗原单克隆抗体3D8A能用于Dot-ELISA检测各期病人血清中循环抗原,并能反映病人感染度与药物治疗效果。

Circulaing antigen in sera from acute, chronic and late stages of schistosomiasis patients was detected by direct dot-ELISA with monoclonal antibody 3D8A against schisto-some gut-associated cathodic antigen linked with peroxidase, the positive rates being 90.6 %, 83.2% and 30.7%, respectively. No positive reactions were found with sera from patients of clonorchiasis, malaria and non-parasitic diseases. The positive rate and the circulating antigen level in EPG>100 group of patients were found to be higher than those in EPG< 100 group. Circulating antigen became negative one year after praziquantel treatment in 84.0% of patients who showed negative fecal examination, while the other patients remained positive with decreasing tilers. The results indicated that the circulating antigen in sera from schistosomiasis patients of various stages can be detected by dot-ELISA with monoclonal antibody 3D8A against circulating schistosome gut-associated cathodic antigen. The authors concluded that the circulating antigen level was correlated with the intensity of infection and the efficacy of treatment.