成人循环内皮祖细胞的性别差异和雌二醇的体外动员作用

Gender differences in adult circulating endothelial progenitor cells(EPCs) and effect of estradiol on arousing of EPCs in vitro

目的:探讨成人循环内皮祖细胞(EPCs)数量及功能的性别差异、女性月经周期对其的影响和雌二醇的体外动员作用。方法:选择月经周期正常的健康女性和年龄匹配的同等条件男性各10名,男性1次采血,女性在月经周期内3个性激素变化阶段(卵泡初期、排卵前期和黄体中期)分别采血。用流式细胞仪计数循环CD34^+/CD133^+/含有激酶插入结构域的受体(KDR)^+的EPCs数量,放射免疫法测定雌二醇水平;经体外培养7 d,计数EPCs,并观察其黏附能力;分别通过跨膜迁移实验和增殖实验观察不同浓度雌二醇的影响。结果:女性组循环EPCs数量明显高于男性组(P<0.01),月经周期内排卵前期和黄体中期明显高于卵泡初期(P<0.05)。经体外培养测定EPCs功能,男性与女性无明显差异。较高浓度的雌二醇(≥1×10^(-9)mol/L)能显著增强EPCs的跨膜迁移能力和增殖能力(P<0.05)。结论:成人循环EPCs数量存在性别差异并且受女性月经周期影响,雌二醇对EPCs的动员有重要作用。

AIM：To investigate the gender differences and influence of menstrual cycle on the number and activity of adult circulating endothelial progenitor cells（EPCs）,and the effect of estradiol on EPCs.METHODS：Ten men and 10 women were enrolled in the study.Peripheral blood samples of the men were collected only once and peripheral blood samples of the women were collected at each menstrual cycle phase（menstrual,pre-ovulatory and mid-luteal phases）. The number of CD34~＋ /CD133~＋ /kinase insert domain-containing receptor（KDR）~＋ EPCs was determined by flow cytometry analysis and the level of circulating estradiol was measured by radioimmunoassay.Mononuclear cells were isolated from the blood and cultured in vitro.After cultured for 7 days,the number and the adhesive capacity of EPCs were observed. The effect of estradiol on the EPCs were detected by transmembrane migration assay and proliferation assay.RESULTS：The number of circulating EPCs was significantly higher in women than that in men（P〈0.01）,and it was higher at the pre-ovulatory phase and the mid-luteal phase than that at the menstrual phase（P〈0.05）.After cultured in vitro,the activity of EPCs did not reveal gender difference.In the cells treated with estradiol at concentration of≥1×10^（-9） mol/L,the capacities of transmembrane migration and proliferation were significantly increased（P〈0.05 ）.CONCLUSION：There are the gender differences of adult circulating EPCs between men and women.The number and activity of adult circulating EPCs may be regulated by menstrual cycle.In addition,estrogen plays an important role in the arousing of EPCs.