微管相关蛋白2和巢蛋白在人胚胎小肠中的表达

Expression of microtubule-associated protein 2 and nestin in small intestine tissues of human embryos

目的:探讨微管相关蛋白2(MAP-2)和巢蛋白(nestin)与人胚胎小肠壁组织发育的关系。方法:应用免疫组织化学PV法和图像分析(NIS-DR)软件检测第2、3、4个月龄段,人胚胎小肠组织内MAP-2和nestin的表达分布规律,数据的组间比较采用单因素方差分析。结果:第2、3、4个月龄段,MAP-2主要表达于人胚胎小肠壁黏膜下和肌间神经丛内神经细胞和纤维,随胎龄的增大,肌间神经丛内神经细胞的阳性强度增强,两两比较,差异有统计学意义(P<0.05)。Nestin蛋白在小肠壁各层均有阳性表达,其黏膜下层和肌间神经丛内阳性细胞数量随胎龄增大而呈先增高再降低的趋势,两两比较,差异有统计学意义(P<0.05)。结论:MAP-2和nestin参与调节人胚胎小肠壁组织的生长发育过程。

AIM：To explore the relationship between expression of microtubule-associated protein 2（MAP -2）/nestin and development of small intestine in human embryos.METHODS：The expression of MAP -2 and nestin proteins was detected in the second,third and fourth months of fetal human embryonic small intestinal tissues by the PV method of immunohistochemistry.The positive cell counts and positive intensities were analyzed with Nikon imaging system （NIS-DR）.One-way ANOVA was applied to compare these positive cell counts and positive intensities.RESULTS： At the second,the third and the fourth month of human embryos,MAP-2 positive expression was observed in the neural cells and neural fibers of the intermuscular nerve plexus and submucosal plexus in the developing small intestines.With the fetal age increasing,the expressive intensity of MAP -2 positive cells in the neural tissues of the intermuscular nerve plexus showed an uptrend.The results of positive intensity values of the MAP-2 positive cells detected in the 3 fetal age periods showed significant difference（P〈0.05）.Nestin positive expression was seen in all layers of the small intestine,and the number of positive cells was first increased and then decreased in the developing small intestines of human embryos with the increasing fetal age.One-way ANOVA results also showed significant difference（P〈0.05 ）.CONCLUSION：MAP-2 and nestin regulate the development of small intestinal tissues in human embryos.