CD70 mRNA及其蛋白在SLE患者T淋巴细胞中的表达

CD70 mRNA and Its Protein in T Lymphocytes of Patients with Systemic Lupus Erythematosus

目的通过研究SLE患者T淋巴细胞CD70mRNA及其蛋白的表达,探讨CD70在SLE发病机理中的作用。方法用定量RT-PCR方法测定15例活动期、15例非活动期SLE患者和15例正常人对照组外周血T淋巴细胞CD70mRNA转录水平。用流式细胞仪检测各组的CD70+CD4+T淋巴细胞阳性率。结果活动期、非活动期SLE患者及正常对照组T淋巴细胞CD70mRNA转录水平分别为(0.82±0.12),(0.73±0.11)和(0.45±0.09),活动期、非活动期SLE患者明显高于正常对照组,差异有统计学意义(P<0.01),且活动期显著高于非活动期SLE患者,差异有统计学意义(P<0.01)。活动期、非活动期SLE患者及正常人外周血CD4+CD70+T淋巴细胞阳性率分别为(80.30±11.04)%,(66.80±3.98)%和(12.48±3.45)%,活动期、非活动期SLE患者明显高于正常对照组,差异有统计学意义(P<0.01),且活动期显著高于非活动期SLE患者,差异有统计学意义(P<0.01)。结论 CD70的过度表达在SLE的发生发展中起着重要的作用,并且CD70过度表达可以作为SLE疾病活动的一个指标。

Objective To detect the expression of CD70 mRNA and protein in T lymphocytes of patients with systemic lupus erythematosus （SLE）. Methods Blood samples were obtained from 15 patients with active SLE, 15 patients with nonactive SLE and 15 normal human controls. Quantitative RT-PCR and flow cytometry were applied to detect the mRNA expression of CDT0 and frequency of CD70＋CD4＋cells respectively. Results Compared with the normal control group ,a significant increase was obse,＇ved in both the expression of CD70 mRNA （P 〈 0.01）and the frequency of CD70＋CD4＋ T lymphocytes （P 〈 0.01） in the other two groups. The expression of CD70 mRNA and the frequency of CDT0＋CD4~ T lymphocytes in the active SLE group were sigificantly higher than those in the nonactive SLE group （P 〈 0.01）. Conclusion The overexpression of CD70 appears to play a significant role in the pathogenesis mechanism of SLE and ,nay act as an indicator of disease activity of SLE.