单克隆抗体Dot-ELISA检测血吸虫病人循环抗原

DOT-ELISA USING MONOCLONAL ANTIBODY FOR DETECTING SCHISTOSOME CIRCULATING ANTIGEN

制备抗日本血吸虫排泄分泌抗原单克隆抗体(McAb),应用于斑点酶联免疫吸咐试验(Dot-ELISA)检测血吸虫病人循环抗原.血吸虫病人血清的阳性率为80.9％(152/188),其中EPG为1～24、25～99和≥100的病人检出率分别为76.8％、86、6％和100％。血吸虫病人经吡喹酮治疗1年以上粪检阴性者50例,均为阴性.并殖吸虫病人、华支睾吸虫病人、丝虫病人和健康人血清均为阴性.McAb-Dot-ELISA操作简便,试剂用量少,肉眼判断结果,可用于血吸虫病的诊断和疗效考核。

Monoclonal antibodies were produced by the fusion of splenic lymphocytes from BALB/c mice immunized with Schistosoma japonicum excretory / secretory antigen and the myeloma cell line SP2/0. The 1B2E7B8 McAb was proved to be specific against the gut antigen of adult worm in IFA. The McAb labelled with HRP was used in Dot-ELISA to detect schistosome circulating antigen.Schistosome circulating antigen was detected in 152 out of 188 proven cases of schis-tosomiasis, accounting for a positive rate of 80.9%. The positive rates for circulating antigen in cases with 1-24, 25-99 and ≥100 EPG were 76.8%, 86.6% and 100% respectively. 10 out of 11 cases who had been checked 2 months after effective treatment became ELISA negative. No circulating antigen was detected in cases with other parasitosis nor in normal individuals. In addition, the McAb-Dot-ELISA showed good reproducibility The results indicated that McAb-Dot-ELISA might be used for diagnosis of schistosomiasis and evaluation of cure.