低毫安的电化学疗法对人乳腺癌MCF-7细胞的细胞周期及c-myc和cyclin E蛋白表达的影响

Effect of Electrochemical Therapy with Low Milli-Ampere on Cell Cycle and Expression of c-myc and Cyclin E in Human Breast Cancer Cell Line MCF-7

目的探讨低毫安(10 m1A)的电化学治疗对体外人乳腺癌MCF-7细胞的细胞周期分布及c-myc和cyclin E表达的影响。方法电化学治疗后培养6h和24h的细胞用MTT法、流式细胞术、免疫组化法测定细胞抑制率、细胞周期分布及细胞c-myc和cyclin E蛋白的表达。结果与对照组相比,治疗组人乳腺癌MCF-7细胞抑制率均随电量增加依次增高(P<0.05);治疗组随电量的增加处于G0/G1期的比例逐渐增高,而S期细胞比例逐渐下降(P<0.05),G2/M期变化不明显(P>0.05);治疗组c-myc和cyclin E表达随电量的增加阳性细胞数逐渐减少。电化学治疗后培养24h比6h各组指标变化显著。结论电化学治疗能通过调节人乳腺癌MCF-7细胞c-myc和cyclin E蛋白的表达,促使细胞G0/G1期阻滞,从而抑制细胞生长。

Objective To explore the effect of electrochemical therapy with low milli - Ampere（ 10 mA） on cell cycle and expression of c - mye and cyclin E in human breast cancer cell line MCF - 7. Methods Equal number of breast cancer cells were treated by the electrochemical treatment, the changes of cell proliferation, cell cycle distribution, c - myc and cyclin E protein expression of MCF - 7 cells were observed by MTY test, flow cytometry and Immunohistochemistry Methods. Results The inhibitory rate was significantly increased after 6h and 24h of ECT tratment compared with the controls（P 〈0.05）. The proportion of G0/G~ phase cells treated with different electrochemical treatment doses in vitro was higher than that in the control group （P 〈 0. 05 ）, but the proport ion of S cells was lower than that in the control group （ P 〈 0.05 ）. The expres- sion o f c - myc and cyclin E protein decreased. Conclusion The study showed that the ECT displayed po- tent antiproliferative activity towards cancer cells through suppressing cell cycle proteins, arresting cell cycle at G0/G1 phase.