摘要
由于叶绿素a外环境极性的不同，其荧光行为有很大的改变。通过小球藻体内叶绿素荧光光谱特性的研究，发现其体内叶绿素的激发波长有在丙酮溶剂中的相同，均为430nm，但发射波长为680nm，红移了13nm。利用小球藻的叶绿素荧光强度进行水体中叶绿素a的定量检测，在浓度为1—100μg/L的浓度范围内，相对荧光强度与叶绿素浓度有很好的相关性，线性方程为Y=0．5338x＋1．8515，线性相关系数R2为0．9974。
The fluorescence characteristics of Chlorophyll a were found to have great change under different polar environment. For the Chlorophyll a in Chlorella vulgaris, its excite wavelength was kept at 430 nm, but 13 nm red-shift was found for its emission wavelength. The fluorescence intensity of Chlorella vulgaris was used for the determination of Chlorophyll a in water, indicating that the response fluorescence intensity and the concentration presented a good linearity in the range of 1 to 100 μg/L, and the linear equation was y = 0.5338x ＋ 1.8515, R2 = 0.9947.
出处
《福建分析测试》
CAS
2012年第2期31-34,共4页
Fujian Analysis & Testing
基金
福建省质量技术监督局项目(No.fjqi 2011025)
关键词
叶绿素A
小球藻
荧光
Chlorophyll a
Chlorella vulgaris
Fluorescence
