Notch1受体和EGFR在颌骨成釉细胞瘤中的表达及其意义

Expression and Significance of Notch1 Receptor and EGFR in Ameloblastoma of the Jaw

目的检测Notch1受体和EGFR在颌骨成釉细胞瘤中的表达,探讨Notch1信号途径和EGFR在颌骨成釉细胞瘤发生中的作用。方法用免疫组化方法检测69例颌骨成釉细胞瘤和5例人牙胚组织标本中Notch1受体和EGFR的表达。结果在牙胚组织中,Notch1受体和EGFR表达于牙源性上皮。所有颌骨成釉细胞瘤标本中均有Notch1受体和EGFR的表达,阳性染色位于细胞膜和细胞质中。高表达的Notch1受体主要分布在外周柱状或立方状肿瘤细胞中,而在中央星网状细胞中Notch1受体低表达。EGFR的表达与Notch1非常相似。结论 Notch1受体和EGFR在牙胚和颌骨成釉细胞瘤中的表达,其表达方式提示Notch1和EGFR在颌骨成釉细胞瘤的发生中起作用,且两条信号通路之间可能存在着"串话"。

Objective To detect the expression of Notchl and EGFR receptor in ameloblastoma of the jaw, and to explore the potential role of Notch signaling pathway and EGFR in this tumor. Methods Tissue specimens of 69 ameloblastomas of the jaw and 5 tooth germs were detected by immunohistoehemistry to determine the expression of Notchl receptor and EGFR. Results In tooth germs, Notehl receptor and EGFR were expressed in odontodenie epithelium. Notehl receptor had a widespread pattern of expression in all amelo- blastoma specimens. Immunostaining was located in cellular membrane and cytoplasm. High expression of Notchl receptor was mainly dis- tributed in peripheral columnar or euboidal neoplastic cells. A low level of Notehl receptor was observed in central polyhedral neoplastic cells. The pattern of epidermal growth factor receptor expression was very similar to Notchl expression. Conclusion The present study demonstrated that Notehl receptor and EGFR were unequivocally expressed in tooth germs and ameloblastoma of the jaw. The pattern of expression revealed that Notchl signaling pathway cross -talk with epidermal growth factor receptor,and they might play a potential role in the tumorigenesis of ameloblastoma.