脂肪干细胞调节创面炎症反应并促进创面愈合

Adipose-derived Stem Cells Modulate Inflammation During Wound Healing and Enhance Wound Repair

目的利用脂肪组织来源的干细胞(adipose-derived stem cells,ADSCs)促进创面愈合并初步探讨其机制。方法采用胶原酶消化GFP转基因小鼠(GFP-C57BL)腹股沟处脂肪组织,获得ADSCs,并在体外证明其具有成骨和成脂的多向分化能力。14只C57BL小鼠随机分为两组并制作创面,实验组注射PBS缓冲液悬浮的ADSCs,对照组注射相同体积的PBS缓冲液。观察并分析创面愈合情况,通过荧光显微镜观察移植细胞的存活情况,利用real-time PCR检测创面组织中促炎因子白细胞介素-1(IL-1)、白细胞介素-6(IL-6)、单核细胞趋化蛋白-1(TCP-1)以及抗炎因子白细胞介素-10(IL-10)的表达。结果分离的细胞具有较强的增殖能力,并具有多向分化潜能。同种异体移植ADSCs可以显著促进创面的愈合速度。移植两周后,荧光显微镜观察创面区域有少量ADSCs存活。与对照组相比,实验组小鼠的创面组织中促炎因子IL-1、IL-6和TCP-1表达下降,抗炎因子IL-10表达上升。结论通过同种异体局部注射ADSCs可以显著促进皮肤创面的愈合,其机制可能与AD-SCs降低了创面局部的炎症反应相关。

Objective To investigate the feasibility of adipose derived stem cells （ADSCs） from GFP - C57BL mouse for wound re- pair and explore the mechanism. Methods ADSCs were isolated from groin adipose tissue of GFP - C57BL mice. The differentiation po- tential of ADSCs was investigated. Fourteen C57BL mice were randomly divided into two groups：experimental group was treated with AD- SCs suspended in PBS, and control group was treated with the same volume of PBS without ADSCs. Wound repair and the survival of AD- SCs were observed and analyzed. Real - time PCR was used to investigate the effect of ADSCs on inflammation during wound repair. Results The cultivated ADSCs had the ability to proliferate continuously and showed characteristics of multi - potent mesenchymal stern cells. Allogeneic transplantation of ADSCs significantly enhanced wound repair. After two weeks of transplantation, a small number of AD- SCs were observed using a fluorescence microscope. ADSCs inhibited the RNA expression of interleukin - 1 （ IL - 1 ） , interleukin - 6 （ IL - 6） and monocyte chemoattractant protein - 1 （TCP - 1） and promoted the RNA expression of IL - 10 in wound area. Conclusion Alloge- neic transplantation of ADSCs could enhance wound repair, which may be due to their regulation effect on inflammation during wound repair.