N-乙酰半胱氨酸拮抗6-羟基多巴胺对骨髓基质细胞的毒性作用

N-acetylcysteine protects bone marrow stromal cells against the toxicity of 6-hydroxydopamine

背景:内源性6-羟基多巴胺能参与氧化应激,N-乙酰半胱氨酸能有效抗氧化和清除自由基。目的:探讨6-羟基多巴胺对骨髓基质细胞的毒性作用及N-乙酰半胱氨酸对其的拮抗作用。方法:在体外培养SD大鼠骨髓基质细胞,取第3代骨髓基质细胞分别加入终浓度为0,0.05,0.1g/L的6-羟基多巴胺和终浓度为0,0.075,0.3,1.2,4.8g/L的N-乙酰半胱氨酸。结果与结论:MTT检测发现0.05和0.1g/L6-羟基多巴胺可以明显降低骨髓基质细胞的细胞活性,而加入6-羟基多巴胺的同时加入0.3g/LN-乙酰半胱氨酸可以显著抑制6-羟基多巴胺的毒性作用。提示抗氧化剂N-乙酰半胱氨酸可以影响6-羟基多巴胺的作用。

BACKGROUND： 6-hydroxydopamine, as an endogenous toxic factor in the pathogenesis of Parkinson＇s disease, participates in oxidative stress. N-acetylcysteine resists oxidation and removes free radicals effectively. OBJECTIVE： To investigate the toxicity of 6-hydroxydopamine in bone marrow stromal cells and the antagonistic effect of N-acetylcysteine on it. METHODS： Bone marrow stromal cells of Sprague-Dawley rats were cultured in vitro. Bone marrow stromal cells of passage 3 were treated with 6-hydroxydopamine with the final concentrations of 0, 0.05, 0.1 g/L and N-acetylcysteine with the final concentrations of 0, 0.075, 0.3, 1.2, 4.8 g/L, respectively. RESULTS AND CONCLUSION： MTT assay showed that 6-hydroxydopamine （0.05 and 0.1 g/L） significantly decreased the viability of bone marrow stromal cells. This toxic effect of 6-hydroxydopamine was significantly inhibited by 0.3 g/L N-acetylcysteine. It suggests that antioxidant N-acetylcysteine may affect the toxic action of 6-hydroxydopamine.