马齿苋多糖对衰老小鼠肠道微生态调节作用的研究

The role of Portulaca oleracea polysaccharide in regulating intestinal micro flora in aged mice

目的探讨中药马齿苋多糖对衰老小鼠微生态调节作用。方法皮下注射D-半乳糖建立衰老小鼠模型,用马齿苋多糖进行治疗,同时设正常对照组、阳性对照组和阴性对照组,用药30 d后处死小鼠,进行肠道微生态指标检测。结果用D-半乳糖造模以后,小鼠肠道双歧杆菌及乳酸杆菌数量降低(P<0.05),而肠杆菌及肠球菌数量增加说明模型建立,血内毒素含量也升高(P<0.05)。用马齿苋治疗后,益生菌数量升高而肠杆菌和肠球菌数量减少,血内毒素含量也降低(P<0.05)。结论马齿苋多糖对衰老小鼠的肠道菌群和血内毒素具有调节作用。

Objective To explore the role Portulaca oleracea polysaccharide （POP） plays in regulating intestinal micro flora in aged mice. Method D-galactose was subcutaneously injected into young mice to induce aging, after which the mice were treated with POP. At the same time, the mice were randomly divided into normal control group, positive control group and negative control group. After 30 days of treatment with various medicines, the intestinal micro flora indexes were determined. Result After 30 days of inducing age in the mice with D-galactose, the number of bifidobacteria and lactobacilli decreased （P 〈 0. 05）, while the number of enterobacter and enterococci increased, indicating that aging was induced in the mice. An increase in blood endotoxin content in the aged group was also observed （P 〈 0. 05）. After treating the aged group with POP, it was observed that there was a reverse in the above indexes （P 〈 0. 05）. Conclusion POP has a regulatory effect on intestinal flora and blood endotoxin content of aged mice.