载银纳米二氧化钛树脂基托对变异链球菌和白色假丝酵母菌抗菌性的体外研究

Study of antibacterial effect of polymethyl methacrylate resin base containing Ag-TiO2 against Streptococcus mutans and Saccharomyces albicans in vitro

目的采用体外法研究载银纳米二氧化钛(Ag-TiO2)树脂基托对口腔常见条件致病菌的单一游离菌体及菌斑生物膜的抗菌性能。方法将不同质量分数的Ag-TiO2抗菌粉剂添加到树脂基托材料中,制成抗菌树脂基托。采用薄膜密贴法分别检测该抗菌树脂基托对变异链球菌、白色假丝酵母菌、金黄色葡萄球菌的抗菌率,并用扫描电镜观察抗菌树脂基托表面变异链球菌、白色假丝酵母菌的生长情况。结果与未添加抗菌剂相比,抗菌剂质量分数为0.7%时,抗菌树脂基托对金黄色葡萄球菌的抗菌率达93.3%;抗菌剂质量分数为1.5%时,抗菌树脂基托对变异链球菌的抗菌率达90.2%;抗菌剂质量分数为2.5%时,抗菌树脂基托对白色假丝酵母菌的抗菌率也达91.2%。与未添加抗菌剂相比,抗菌树脂基托表面变异链球菌、白色假丝酵母菌的黏附数量明显减少。结论Ag-TiO2可以显著提高树脂基托的抗菌性能。

Objective To study the antibacterial effect of polymethyl methyacrylate（PMMA） resin base containing Ag-TiO2 antibacterial agent against common conditioned pathogen and their bacterial plaque in vitro.Methods Diffe-rent qualities of Ag-TiO2 antibacterial agent were added into PMMA resin base in order to prepare antibacterial PMMA resin base.Then,in vitro analysis of antibacterial effect of the resin base against Streptococcus mutans,Staphylococcus aureus,Saccharomyces albicans were assayed with the pellicle-sticking method.Furthermore,the growth of Streptococ-cus mutans and Saccharomyces albicans bacterial plaque on the antibacterial PMMA resin base were examined by scanning electron microscope.Results The inhibition percent for Staphylococcus aureus was 93.3% by antibacterial PMMA resin base with 0.7% Ag-TiO2;for Streptococcus mutans 90.2% with 1.5% Ag-TiO2;for Saccharomyces albi-cans 91.2% with 2.5% Ag-TiO2.Bacterial plaque of Streptococcus mutans and Saccharomyces albicans on antibacterial resin base were inhibited effectively.Conclusion The antibacterial property of the PMMA resin base is effectively enhanced with Ag-TiO2 antibacterial agent.