人参皂苷Rg1对血小板聚集及环磷腺苷的影响

The effect of rat platelet aggregation and platelet cyclic AMP metabolism by Ginsenoside Rg1

[目的]探讨三七活血有效成分人参皂苷Rg1对大鼠血小板聚集的影响。[方法]体外分离大鼠血小板,采用比浊法观察不同浓度Rg1(0.5、1.5、2.5、3、4 mmol/L,)对体外大鼠二磷酸腺苷(ADP)诱导的血小板聚集的抑制作用,以阳性药阿司匹林作为对照;采用酶联免疫(ELISA)法检测Rg1对大鼠血小板环磷腺苷(cAMP)的影响。[结果]0.5 mmol/L人参皂苷Rg1组对血小板聚集无明显影响;阿司匹林组及人参皂苷Rg1(4、3、2.5、1.5 mmol/L)组最大聚集率均明显小于阴性对照组(P<0.01),其抑制率分别为18.95%,59.04%,40.37%,29.77%,9.77%;不同浓度Rg1组对ADP诱导后血小板cAMP的含量与ADP组没有显著性差异。[结论]Rg1可明显抑制ADP诱导的血小板聚集,但其机制并不是通过升高血小板内cAMP含量而抑制血小板激活。

[Objective] To explore the effective components of ginsenoside Rgl on rat platelet aggregation and cAMP. [Method] Using turbidimetry in different concentration of Rgl （0.5,1.5,2.5,3,4 retool/L） of rats in vitro induced inhibition of platelet aggregation, to positive drug aspirin was used as control; to study the content of cAMP on rat platelet by Rgl.[Results] 0.5 mmol/L ginseng saponins group Rgl on platelet aggregation effect; aspirin group and ginsenoside Rgl （ 4,3,2.5,1.5 mmol/L ） group maximal aggregation ratio were significantly smaller than the negative control group （P〈0.01）, the inhibition rates were 18.95% ,59.04% ,40.37% ,29.77% ,9.77%; Rgl of different concentration groups on ADP after induction of platelet cAMP content and ADP group were not significant difference. [Conclusion] Rgl can inhibit platelet activation, but not by elevating the content of rat platelet.