松质骨基质材料应用于牙周组织工程的可行性

Feasibility of cancellous bone matrix in periodontal tissue engineering

背景:松质骨基质材料具有骨诱导性和骨传导性双重特性,已被成功应用于骨再生及骨组织工程研究。目的:分析松质骨基质材料的细胞相容性与毒性,探讨其应用于牙周组织工程的可行性。方法:采用改良组织块法体外培养人牙周膜细胞,将其接种于松质骨基质三维支架上复合培养,采用细胞计数方法和扫描电镜观察人牙周膜细胞在松质骨基质支架上的附着、生长情况,并通过MTT测试法和碱性磷酸酶活性检测法观察松质骨基质浸提液对人牙周膜细胞增殖及功能表达的影响。结果与结论:扫描电镜可见松质骨基质具有良好的多孔网状结构,人牙周膜细胞在松质骨基质上贴附紧密,生长旺盛,伸展充分,而人牙周膜细胞在不同浓度材料浸提液中的生长、增殖及碱性磷酸酶活性与阴性对照组相比差异无显著性意义。表明松质骨基质具有良好的三维空间结构和细胞相容性,且无细胞毒性,有望应用于牙周组织再生及牙周组织工程研究。

BACKGROUND： Cancellous bone matrix （CBM） has been proved to be valuable in the bone regeneration and bone tissue engineering based on its double characteristics of bone induction and bone conduction. OBJECTIVE： To analyze the cytocompatibility and cytotoxicity of CBM in order to evaluate the feasibility of CBM application in periodontal tissue engineering. METHODS： Human periodontal ligament cells （PDLCs） cultured with modified-tissue block method in vitro were collected and seeded on three-dimensional framework of CBM. The growth and adhesion of PDLCs on the scaffolds were observed by cell counting and scanning electronic microscope. And the influences of the CBM extracts on cell proliferation and differentiation were observed by MTT assay and alkaline phosphatase activity assay. RESULTS AND CONCLUSION： Cells adhered and proliferated on the three-dimensional framework of CBM, and scanning electronic microscope showed the porous structure of CBM and eugonic growth of cells on the scaffolds. The differences in cell proliferation and differentiation of different concentrations of CBM extracts had no significance compared with the negative control group. It is indicated that CBM has a good three-dimensional structure and cytocompatibility and has no cytotoxicity, and CBM is expected to be used in periodontal tissue regeneration and engineering.