外源性单磷酸鸟苷环二聚体对变形链球菌基因表达的影响

Effects of exogenous cyclic dimeric guanosinemonophosphate on the gene expression of streptococcus mutans

背景:前期研究证实变形链球菌内部存在单磷酸鸟苷环二聚体信号通路,该通路介导变形链球菌的生物膜形成及在体外牙釉质表面的黏附。目的:以基因芯片技术分析单磷酸鸟苷环二聚体对变形链球菌生物学特性的影响。方法:以外源性单磷酸鸟苷环二聚体干预变形链球菌UA159,提取其总RNA,并以标准菌株的总RNA作为对照,与变形链球菌全基因组芯片杂交,筛选差异基因。结果与结论:外源性单磷酸鸟苷环二聚体干预后,变形链球菌中glgA、glgB、msmF、gftA、lacG、lepB、rgpAc、bacC、apt69个基因表达上调,Hrc、ProC、HprT、Pdp、Glk、cdsA共6个基因表达下调。所获得的差异基因主要与细胞趋向性和生物膜形成、信号转导、糖代谢、等途径相关。说明单磷酸鸟苷环二聚体影响变形链球菌的致龋性。

BACKGROUND： The preliminary study has confirmed the existence of cyclic dimeric guanosinemonophosphate （c-di-GMP） signaling pathway in streptococcus mutans. The pathway mediates the streptococcus mutans biofilm formation and in vitro adhesion to enamel surface. OBJECTIVE： To analysis the effects of c-di-GMP on the biological properties of streptococcus mutans by gene chip technology. METHODS： Exogenous c-di-GMP was used to interfere streptococcus mutans UA159. The total RNA was extracted, the total RNA of standard strain was as control, and then hybridized with streptococcus mutans whole-genome chips. The different genes were filtered. RESULTS AND CONCLUSION： Expressions of 9 genes （glgA, glgB, msmF, gftA, lacG, lepB, rgpAc, bacC, apt6） were up-regulated while six genes （Hrc, ProC, HprT, Pdp, Glk, cdsA） expressions were down-regulated after exogenous c-di-GMP interfering. Differences genes were mainly related to the biological characteristics, such as cell tropism, biofilm formation, signal transduction, and energy metabolism. It showed that the c-di-GMP could affect the cariogenic ability of streptococcus mutans.