微载体培养技术在骨与软骨组织工程研究中的应用

Application of microcarrier culture techniques in bone and cartilage tissue engineering

背景:骨与软骨组织工程学中增殖种子细胞和保持细胞特定表型是其难点,微载体生物反应器培养系统提供了很好的条件来解决这个问题。目的:分析近年来国内外骨、软骨细胞微载体培养的研究进展,为骨与软骨细胞微载体培养技术和组织工程研究提供理论基础。方法:由第一作者在2010-11进行检索。检索数据库:PubMed数据库(网址http://www.ncbi.nlm.gov/PubMed);万方数据库(网址http://www.wanfangdata.com.cn),资料的检索时间范围为1967/2011。英文检索词为"microcarrier,cartilage,tissue engineering",中文检索词为"微载体,软骨,组织工程学"。排除与本文无关及陈旧、重复的文章,共保存32篇文献做进一步分析。结果与结论:在微载体培养系统中,可较好的调控骨与软骨细胞培养条件,能在短时间内大量的增殖,并能保持其细胞的表型,甚至出现表型增强现象,在骨、软骨组织工程学研究和临床应用中有着巨大潜力。

BACKGROUND： Proliferation and specific phenotype maintaining of seed cells are the difficulties in bone and cartilage tissue engineering. The microcarrier bioreactor culture system provides a good method to solve this problem. OBJECTIVE： To analyze the domestic and international development of microcarrier culture of bone and cartilage cells, and to provide the theoretical basis for microcarrier culture of bone and cartilage cells and researches in tissue engineering. METHODS： A computer-based online search of articles published from 1967 to 2011was performed at November 2010 in PubMed database （http：//www.ncbi.nlm.gov/PubMed） and Wanfang database （http：//www.wanfangdata.com.cn） using the key words of ＂microcarrier, cartilage, tissue engineering＂ in English and in Chinese, respectively. Articles irrelative to this paper, antiquated or repeated literatures were excluded. Totally 32 articles were chosen for further analysis. RESULTS AND CONCLUSION： Culture conditions of bone and cartilage cells in microcarrier bioreactor culture system can be well regulated to achieve a large number of proliferation and phenotype maintaining of the cells, or even achieve an enhancement of phenotype. This technology has a good potential application in bone and cartilage tissue engineering and clinical work.