PCR-DGGE指纹图谱技术分析2型糖尿病模型小鼠胃微生物菌群结构

Analysis of the Bacterial Community in Gastric Samples from Mice with TypeⅡDiabetes by Using PCR-DGGE Fingerprint

糖尿病患者多出现胃肠道功能紊乱,如急性胃炎、胃溃疡,以及胃动力低下,胃排空延迟、胃内细菌过度滋长等,进一步导致肠道疾病。研究糖尿病胃内容物菌群结构变化对研究糖尿病发病机理及并发症治疗具有重要意义。该项研究采用变性梯度凝胶电泳技术,对10只2型糖尿病模型小鼠及10只正常对照小鼠进行胃内容物和粘膜样本菌群结构研究。结果表明,实验组小鼠与对照组小鼠胃内容物和粘膜菌群条带数、多样性指数、丰富度指数、均匀度指数与优势度指数均无显著差异,且相似度系数差异不明显。而特异条带测序结果显示正常小鼠胃内含乳杆菌,实验组小鼠胃内乳杆菌含量很低甚至检测不到。提示胃内乳杆菌与2型糖尿病密切相关。

Gastrointestinal complications are common in patients with diabetes mellitus which involve the acute gastritis and gastric ulcer.Because gastric dyskinesis,delayed gastric emptying,gastric bacterial over-growth,these will further lead to intestinal diseases.Research on the change in diabetes stomach content bacterium group structure,and it＇is important for the study of diabetes pathogenesis and the treatment of complications.Bacterial DNAs from 10 type 2 diabetes mice and 10 normal control mice were extracted from gastric contents and gastric mucosal samples and then characterized by PCR-denaturing gradient gel electrophoresis（DGGE）.After DGGE profilings was obtained,the diversity and similarity analyses were carried out by the number of band,Shannon-Weaver（H＇）,Margalef index（R）,Pielou index（E） and Simpson index（D）,cluster analysis and cloning sequencing.The results show that there was no significant difference in Shannon-Weaver diversity index、Margalef index 、Pielou index、and Simpson index between experimental group and control group.There is no significant distinction in the Dice similarity coefficient（Cs） between the two groups.The result of sequencing illustrates that Lactobacillus is in the gastric of normal control mice,but type 2 diabetes mice.These results suggest that lactobacillus is closely related to type 2 diabetes.